ArticlePlant cell reports2010
Agrobacterium tumefaciens-mediated transformation of Withania somnifera (L.) Dunal: an important medicinal plant.
Article in Plant cell reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Improving transformation and regeneration efficiency in medicinal plants: insights from other recalcitrant species.Journal of experimental botany · 2025Review
- Agrobacterium tumefaciens-Mediated Plant Transformation: A Review.Molecular biotechnology · 2024Review
- Critical factors influencing in vitro propagation and modulation of important secondary metabolites inPlant cell, tissue and organ culture · 2022Review
- Plant regeneration and transformation of Trachyspermum ammi using Agrobacterium tumefaciens and zygotic embryos.Journal, genetic engineering & biotechnology · 2021Article
- Article
- Elite hairy roots of3 Biotech · 2018Article
- Article
- Addressing Challenges to Enhance the Bioactives ofBioMed research international · 2017Review
- Review
- Ectopic overexpression of WsSGTL1, a sterol glucosyltransferase gene in Withania somnifera, promotes growth, enhances glycowithanolide and provides tolerance to abiotic and biotic stresses.Plant cell reports · 2016Article
- Comparative interactions of withanolides and sterols with two members of sterol glycosyltransferases from Withania somnifera.BMC bioinformatics · 2015Article
- Article
- RNAi-mediated gene silencing of WsSGTL1 in W.somnifera affects growth and glycosylation pattern.Plant signaling & behavior · 2015Article
- Article
- Efficient genetic transformation of Withania coagulans (Stocks) Dunal mediated by Agrobacterium tumefaciens from leaf explants of in vitro multiple shoot culture.Protoplasma · 2013Article
- Cloning and functional characterization of 3-hydroxy-3-methylglutaryl coenzyme A reductase gene from Withania somnifera: an important medicinal plant.Protoplasma · 2013Article
- Cloning and characterization of 2-C-methyl-D-erythritol-4-phosphate pathway genes for isoprenoid biosynthesis from Indian ginseng, Withania somnifera.Protoplasma · 2013Article
- High-efficiency Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) and regeneration of insect-resistant transgenic plants.Plant cell reports · 2011Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This report describes Agrobacterium tumefaciens-mediated transformation of Withania somnifera--an important Indian medicinal plant. A. tumefaciens strain LBA4404, containing the binary vector pIG121Hm was used for transformation, along with the gusA reporter gene with intron under the transcriptional control of the Cauliflower Mosaic Virus (CaMV) 35S promoter. The leaf segments from two-and-a-half-month-old green house-grown seedlings were more efficient in transformation, as compared to those from the in vitro-grown shoots. Second expanded leaf from the shoot tip gave the highest transient transformation efficiency. Selection of transgenic shoots was done in the presence of 50 mg l(-1) kanamycin. Polymerase chain reaction analysis of T(0) transgenic plants showed the presence of gusA and nptII genes. The expression of these transgenes in T(1) progeny was confirmed by RT-PCR. The integration of gusA gene was confirmed by Southern blot analysis. The transformation efficiency was found to be 1.67%.
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Registered trials
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