ArticleThe Biochemical journal1991
Insulin secretory granule biogenesis. Co-ordinate regulation of the biosynthesis of the majority of constituent proteins.
Article in The Biochemical journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 82 citations in OpenAlex.
- Sustained hyperglycemia specifically targets translation of mRNAs for insulin secretion.The Journal of clinical investigation · 2023Article
- The making of insulin in health and disease.Diabetologia · 2020Review
- β-Cell Control of Insulin Production During Starvation-Refeeding in Male Rats.Endocrinology · 2018Article
- The dynamic plasticity of insulin production in β-cells.Molecular metabolism · 2017Review
- Whole Genome Sequencing Identifies a Novel Factor Required for Secretory Granule Maturation in Tetrahymena thermophila.G3 (Bethesda, Md.) · 2016Article
- Pancreatic β-Cell Adaptive Plasticity in Obesity Increases Insulin Production but Adversely Affects Secretory Function.Diabetes · 2016Article
- Secretion of Fc-amidated peptide fusion proteins by Chinese hamster ovary cells.BMC biotechnology · 2015Article
- INS-gene mutations: from genetics and beta cell biology to clinical disease.Molecular aspects of medicine · 2015Review
- Regulation of β-cell function by RNA-binding proteins.Molecular metabolism · 2013Review
- Islet autoantigens: structure, function, localization, and regulation.Cold Spring Harbor perspectives in medicine · 2012Review
- Translational control of glucose-induced islet amyloid polypeptide production in pancreatic islets.Endocrinology · 2012Article
- Rapid changes of mRNA-binding protein levels following glucose and 3-isobutyl-1-methylxanthine stimulation of insulinoma INS-1 cells.Molecular & cellular proteomics : MCP · 2009Article
- Distinct glucose-dependent stress responses revealed by translational profiling in pancreatic beta-cells.The Journal of endocrinology · 2007Article
- New class of cargo protein in Tetrahymena thermophila dense core secretory granules.Eukaryotic cell · 2002Article
- Preptin derived from proinsulin-like growth factor II (proIGF-II) is secreted from pancreatic islet beta-cells and enhances insulin secretion.The Biochemical journal · 2001Article
- Identification and characterization of pancreatic eukaryotic initiation factor 2 alpha-subunit kinase, PEK, involved in translational control.Molecular and cellular biology · 1998Article
- Tetrahymena: the key to the genetic analysis of the regulated pathway of polypeptide secretion?Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Insulin secretory granule biogenesis and the proinsulin-processing endopeptidases.Diabetologia · 1994Review
- Sorting and processing of secretory proteins.The Biochemical journal · 1994Review
- Control of insulin gene expression by glucose.The Biochemical journal · 1992Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Two-dimensional gel-electrophoretic analysis combined with fluorography and densitometric quantification was used to examine the effects of glucose on the biosynthesis of rat pancreatic islet proteins. An increase in the medium glucose concentration from 2.8 to 16.7 mM produced a 10-20 fold stimulation in the synthesis of 10 out of 260 detected islet proteins, as judged by incorporation of [35S]methionine during a 20 min incubation. The synthetic rates of the majority of the remaining proteins were stimulated by 2-4-fold. Greater resolution achieved by pulse-chase labelling and subcellular fractionation showed that, of 32 major proteins localized to insulin secretory granules, the biosynthesis of 25 were stimulated 15-30-fold by glucose. By contrast, only eight of 160 proteins in the soluble fraction showed a response of similar magnitude. It is concluded that there is a major and co-ordinated activation of the biosyntheses of proteins destined for secretory granules, which most likely occurs at the level of translational initiation and signal-recognition-particle-mediated translocation into the endoplasmic reticulum lumen. However, it is clear that not all granule proteins, or the majority of proteins translocated across the endoplasmic reticulum membrane, are affected in an equivalent manner. In addition, the synthesis of a small number of cytosolic proteins may be increased markedly by insulinotropic stimuli.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.