ArticleMethods in molecular biology (Clifton, N.J.)2010
2-aminopurine as a probe for quadruplex loop structures.
Article in Methods in molecular biology (Clifton, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences.Nucleic acids research · 2022Article
- Studies on the Interactions of 3,11-Difluoro-6,8,13-trimethyl-8Molecules (Basel, Switzerland) · 2021Article
- Linear consecutive hexaoxazoles as G4 ligands inducing chair-type anti-parallel topology of a telomeric G-quadruplex.RSC advances · 2020Article
- Two-quartet kit* G-quadruplex is formed via double-stranded pre-folded structure.Nucleic acids research · 2019Article
- Triplex-quadruplex structural scaffold: a new binding structure of aptamer.Scientific reports · 2017Article
- The Consequences of Overlapping G-Quadruplexes and i-Motifs in the Platelet-Derived Growth Factor Receptor β Core Promoter Nuclease Hypersensitive Element Can Explain the Unexpected Effects of Mutations and Provide Opportunities for Selective Targeting of Both Structures by Small Molecules To Downregulate Gene Expression.Journal of the American Chemical Society · 2017Article
- Alternative DNA structure formation in the mutagenic human c-MYC promoter.Nucleic acids research · 2017Article
- Folding and unfolding pathways of the human telomeric G-quadruplex.Journal of molecular biology · 2014Article
- Quadruplex formation as a molecular switch to turn on intrinsically fluorescent nucleotide analogs.Nucleic acids research · 2013Article
- Populated intermediates in the thermal unfolding of the human telomeric quadruplex.Journal of the American Chemical Society · 2012Article
- Cooperative cluster formation, DNA bending and base-flipping by O6-alkylguanine-DNA alkyltransferase.Nucleic acids research · 2012Article
- G-quadruplex structure and stability illuminated by 2-aminopurine phasor plots.Nucleic acids research · 2012Article
- Isothermal folding of G-quadruplexes.Methods (San Diego, Calif.) · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescent reporter groups have served for many years as sensitive probes of macromolecular structure. Such probes can be especially useful in comparative studies such as detection of conformational changes and discrimination among structural models. Spectroscopic methods such as fluorescence are attractive because they are rapid, require small amounts of material, are nondestructive, can be carried out with commonly available equipment, and are relatively inexpensive. In addition, there is a rich library of theoretical and practical materials available to aid in data interpretation.The intrinsic fluorescence of most nucleic acids is too low to be useful in structural studies. Thus, it is necessary to incorporate a suitable reporter group to utilize fluorescence methods involving polynucleotide structure. A highly fluorescent adenine analog, 2-aminopurine, has long served in this capacity. The present article describes our use of 2-aminopurine as a probe of loop structures in quadruplex DNA. In particular, we show how knowledge of the relative intensity of 2-aminopurine emission as well as its sensitivity to exogenous quenching molecules such as acrylamide can aid in comparing crystal and solution structures of an oligonucleotide model of the human telomere and in discrimination among models containing tandem repeats of the telomeric quadruplex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.