ArticleJournal of molecular evolution2009
Evolutionary analysis of mammalian enamelin, the largest enamel protein, supports a crucial role for the 32-kDa peptide and reveals selective adaptation in rodents and primates.
Article in Journal of molecular evolution, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 54 citations in OpenAlex.
- Association of LTF, ENAM, and AMELX polymorphisms with dental caries susceptibility: a meta-analysis.BMC oral health · 2020Pooled it
- Phylogenetic Signal in Primate Tooth Enamel Proteins and its Relevance for Paleoproteomics.Genome biology and evolution · 2025Article
- Ganoin and acrodin formation on scales and teeth in spotted gar: A vital role of enamelin in the unique process of enamel mineralization.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2023Article
- Coemergence of the Amphipathic Helix on Ameloblastin With Mammalian Prismatic Enamel.Molecular biology and evolution · 2022Article
- AMELX and ENAM Polymorphisms and Dental Caries.International journal of dentistry · 2022Article
- Molecular Evolution of Tooth-Related Genes Provides New Insights into Dietary Adaptations of Mammals.Journal of molecular evolution · 2021Article
- ENAM mutations and digenic inheritance.Molecular genetics & genomic medicine · 2019Article
- Association between ENAM polymorphisms and dental caries in children.Saudi journal of biological sciences · 2019Article
- Mapping the Tooth Enamel Proteome and Amelogenin Phosphorylation Onto Mineralizing Porcine Tooth Crowns.Frontiers in physiology · 2019Article
- Evolutionary Analysis of the Mammalian Tuftelin Sequence Reveals Features of Functional Importance.Journal of molecular evolution · 2017Article
- Article
- Evolutionary Analysis Predicts Sensitive Positions of MMP20 and Validates Newly- and Previously-IdentifiedFrontiers in physiology · 2017Article
- Human enamel thickness and ENAM polymorphism.International journal of oral science · 2016Article
- Evolutionary analysis of selective constraints identifies ameloblastin (AMBN) as a potential candidate for amelogenesis imperfecta.BMC evolutionary biology · 2015Article
- Article
- Amelotin: an enamel matrix protein that experienced distinct evolutionary histories in amphibians, sauropsids and mammals.BMC evolutionary biology · 2015Article
- Molecular evolution of the tissue-nonspecific alkaline phosphatase allows prediction and validation of missense mutations responsible for hypophosphatasia.The Journal of biological chemistry · 2014Article
- FAM20C functions intracellularly within both ameloblasts and odontoblasts in vivo.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2013Article
- Dental enamel development: proteinases and their enamel matrix substrates.ISRN dentistry · 2013Review
- Localization and quantitative co-localization of enamelin with amelogenin.Journal of structural biology · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enamelin (ENAM) plays an important role in the mineralization of the forming enamel matrix. We have performed an evolutionary analysis of mammalian ENAM to identify highly conserved residues or regions that could have important function (selective pressure), to predict mutations that could be associated with amelogenesis imperfecta in humans, and to identify possible adaptive evolution of ENAM during 200 million years ago of mammalian evolution. In order to fulfil these objectives, we obtained 36-ENAM sequences that are representative of the mammalian lineages. Our results show a remarkably high conservation pattern in the region of the 32-kDa fragment of ENAM, especially its phosphorylation, glycosylation, and proteolytic sites. In primates and rodents we also identified several sites under positive selection, which could indicate recent evolutionary changes in ENAM function. Furthermore, the analysis of the unusual signal peptide provided new insights on the possible regulation of ENAM secretion, a hypothesis that should be tested in the near future. Taken together, these findings improve our understanding of ENAM evolution and provide new information that would be useful for further investigation of ENAM function as well as for the validation of mutations leading to amelogenesis imperfecta.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.