ArticlePloS one2009
Primordial germ cell-like cells differentiated in vitro from skin-derived stem cells.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 86 citations in OpenAlex.
- Transcriptome-Wide Sequencing Identifies Non-Coding RNAs and Their Competing Endogenous RNA Networks During the Stages of pPGCLCs-Induced Differentiation.Stem cell reviews and reports · 2025Article
- Co-culture with adipose mesenchymal stem cells promotes Blastocyst formation and gene expression in embryos from aged mice.Regenerative therapy · 2025Article
- Emerging Contributions of Pluripotent Stem Cells to Reproductive Technologies in Veterinary Medicine.Journal of developmental biology · 2024Review
- Prevention of methylmercury-triggered ROS-mediated impairment of embryo development by co-culture with adult adipose-derived mesenchymal stem cells.Toxicology research · 2024Article
- Transcriptomic landscape reveals germline potential of porcine skin-derived multipotent dermal fibroblast progenitors.Cellular and molecular life sciences : CMLS · 2023Article
- Murine skin-derived multipotent papillary dermal fibroblast progenitors show germline potential in vitro.Stem cell research & therapy · 2023Article
- Conservation Biology and Reproduction in a Time of Developmental Plasticity.Biomolecules · 2022Review
- The foundational framework of tumors: Gametogenesis, p53, and cancer.Seminars in cancer biology · 2022Review
- YAP regulates porcine skin-derived stem cells self-renewal partly by repressing Wnt/β-catenin signaling pathway.Histochemistry and cell biology · 2022Article
- The proliferation role of LH on porcine primordial germ cell-like cells (pPGCLCs) through ceRNA network construction.Clinical and translational medicine · 2021Article
- In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded.Cells · 2020Review
- The role of microRNAs in embryonic stem cell and induced pluripotent stem cell differentiation in male germ cells.Journal of cellular physiology · 2019Review
- Isolation and Culture of Skin-Derived Differentiated and Stem-Like Cells Obtained from the Arabian Camel (Animals : an open access journal from MDPI · 2019Article
- Human Ovarian Theca-Derived Multipotent Stem Cells Have The Potential to Differentiate into Oocyte-Like Cells In Vitro.Cell journal · 2019Article
- Retinoic acid enhances germ cell differentiation of mouse skin-derived stem cells.Journal of ovarian research · 2018Article
- Revolutionize livestock breeding in the future: an animal embryo-stem cell breeding system in a dish.Journal of animal science and biotechnology · 2018Review
- Review
- Starvation at birth impairs germ cell cyst breakdown and increases autophagy and apoptosis in mouse oocytes.Cell death & disease · 2017Article
- Skin-derived stem cells as a source of primordial germ cell- and oocyte-like cells.Cell death & disease · 2016Review
- Increasing of blastocyst rate and gene expression in co-culture of bovine embryos with adult adipose tissue-derived mesenchymal stem cells.Journal of assisted reproduction and genetics · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundWe have previously demonstrated that stem cells isolated from fetal porcine skin have the potential to form oocyte-like cells (OLCs) in vitro. However, primordial germ cells (PGCs), which must also be specified during the stem cell differentiation to give rise to these putative oocytes at more advanced stages of culture, were not systematically characterized. The current study tested the hypothesis that a morphologically distinct population of cells derived from skin stem cells prior to OLC formation corresponds to putative PGCs, which differentiate further into more mature gametes. METHODOLOGY/PRINCIPAL
findingsWhen induced to differentiate in an appropriate microenvironment, a subpopulation of morphologically distinct cells, some of which are alkaline phosphatase (AP)-positive, also express Oct4, Fragilis, Stella, Dazl, and Vasa, which are markers indicative of germ cell formation. A known differentially methylated region (DMR) within the H19 gene locus, which is demethylated in oocytes after establishment of the maternal imprint, is hypomethylated in PGC-like cells compared to undifferentiated skin-derived stem cells, suggesting that the putative germ cell population undergoes imprint erasure. Additional evidence supporting the germ cell identity of in vitro-generated PGC-like cells is that, when labeled with a Dazl-GFP reporter, these cells further differentiate into GFP-positive OLCs. SIGNIFICANCE: The ability to generate germ cell precursors from somatic stem cells may provide an in vitro model to study some of the unanswered questions surrounding early germ cell formation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.