ArticleIntegrative biology : quantitative biosciences from nano to macro2009
Cellular observations enabled by microculture: paracrine signaling and population demographics.
Article in Integrative biology : quantitative biosciences from nano to macro, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed.
- Insights on Three Dimensional Organoid Studies for Stem Cell Therapy in Regenerative Medicine.Stem cell reviews and reports · 2024Review
- Macro- and micro-scale culture environment differentially regulate the effects of crowding on macrophage function.Biotechnology and bioengineering · 2024Article
- Review
- Toward improvedAPL bioengineering · 2021Article
- Induced Pluripotent Stem Cells on a Chip: A Self-Contained, Accessible, Pipette-less iPSC Culturing and Differentiation Kit.SLAS technology · 2021Article
- Modeling chemical effects on breast cancer: the importance of the microenvironment in vitro.Integrative biology : quantitative biosciences from nano to macro · 2020Review
- Cell patterning by surface tension pinning in microfluidic channels.Biomicrofluidics · 2020Article
- Monitoring of Microphysiological Systems: Integrating Sensors and Real-Time Data Analysis toward Autonomous Decision-Making.ACS sensors · 2019Article
- Measuring and regulating oxygen levels in microphysiological systems: design, material, and sensor considerations.The Analyst · 2019Review
- GPCRs in Autocrine and Paracrine Regulations.Frontiers in endocrinology · 2019Review
- Open multi-culture platform for simple and flexible study of multi-cell type interactions.Lab on a chip · 2018Article
- Double-exclusive liquid repellency (double-ELR): an enabling technology for rare phenotype analysis.Lab on a chip · 2018Article
- Human iPSC-Derived Endothelial Cells and Microengineered Organ-Chip Enhance Neuronal Development.Stem cell reports · 2018Article
- Adaptation of a Simple Microfluidic Platform for High-Dimensional Quantitative Morphological Analysis of Human Mesenchymal Stromal Cells on Polystyrene-Based Substrates.SLAS technology · 2017Article
- Tumor Microenvironment on a Chip: The Progress and Future Perspective.Bioengineering (Basel, Switzerland) · 2017Review
- Transitions from mono- to co- to tri-culture uniquely affect gene expression in breast cancer, stromal, and immune compartments.Biomedical microdevices · 2016Article
- Effects of substrate stiffness and cell-cell contact on mesenchymal stem cell differentiation.Biomaterials · 2016Article
- Single cell functional analysis of multiple myeloma cell populations correlates with diffusion profiles in static microfluidic coculture systems.Biomicrofluidics · 2016Article
- Progress towards understanding heterotypic interactions in multi-culture models of breast cancer.Integrative biology : quantitative biosciences from nano to macro · 2016Review
- Microbial metabolomics in open microscale platforms.Nature communications · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
The cellular microenvironment plays a critical role in shaping and directing the process of communication between the cells. Soluble signals are responsible for many cellular behaviors such as cell survival, proliferation and differentiation. Despite the importance of soluble signals, canonical methods are not well suited to the study of soluble factor interactions between multiple cell types. Macro-scale technology often puts cells into a convective environment that can wash away and dilute soluble signals from their targets, minimizing local concentrations of important factors. In addition, current methods such as transwells, require large numbers of cells and are limited to studying just two cell types. Here, we present data supporting the use of microchannels to study soluble factor signaling providing improved sensitivity as well as the ability to move beyond existing co-culture and conditioned medium paradigms. In addition, we present data suggesting that microculture can be used to unmask effects of population demographics. In this example the data support the hypothesis that a growth promoting subpopulation of cells exists in the mouse mammary gland.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.