ArticleCellular and molecular bioengineering2008
Dependence of zonal chondrocyte water transport properties on osmotic environment.
Article in Cellular and molecular bioengineering, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- Intrinsic Viscoelasticity of Type II Collagen Contributes to the Viscoelastic Response of Immature Bovine Articular Cartilage Under Unconfined Compression Stress Relaxation.Journal of biomechanical engineering · 2026Article
- Osmolarity-Induced Altered Intracellular Molecular Crowding Drives Osteoarthritis Pathology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Modulation of sirtuins during monolayer chondrocyte culture influences cartilage regeneration upon transfer to a 3D culture environment.Frontiers in bioengineering and biotechnology · 2022Article
- Direct Osmotic Pressure Measurements in Articular Cartilage Demonstrate Nonideal and Concentration-Dependent Phenomena.Journal of biomechanical engineering · 2021Article
- Importance of Osmolarity and Oxygen Tension for Cartilage Tissue Engineering.BioResearch open access · 2020Article
- Epistasis of polymorphisms related to the articular cartilage extracellular matrix in knee osteoarthritis: Analysis-based multifactor dimensionality reduction.Genetics and molecular biology · 2020Article
- Finite Element Formulation of Multiphasic Shell Elements for Cell Mechanics Analyses in FEBio.Journal of biomechanical engineering · 2018Article
- Raman spectroscopic imaging for quantification of depth-dependent and local heterogeneities in native and engineered cartilage.NPJ Regenerative medicine · 2018Article
- Wear and damage of articular cartilage with friction against orthopedic implant materials.Journal of biomechanics · 2015Article
- Physicochemical and biomechanical stimuli in cell-based articular cartilage repair.Current rheumatology reports · 2015Review
- Promoting increased mechanical properties of tissue engineered neocartilage via the application of hyperosmolarity and 4α-phorbol 12,13-didecanoate (4αPDD).Journal of biomechanics · 2014Article
- Simultaneous magnetic resonance imaging and consolidation measurement of articular cartilage.Sensors (Basel, Switzerland) · 2014Article
- TRPV4-mediated mechanotransduction regulates the metabolic response of chondrocytes to dynamic loading.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Applied osmotic loading for promoting development of engineered cartilage.Journal of biomechanics · 2013Article
- Osmotic challenge drives rapid and reversible chromatin condensation in chondrocytes.Biophysical journal · 2013Article
- Trimethylamine N-oxide as a media supplement for cartilage tissue engineering.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2012Article
- Transport phenomena in articular cartilage cryopreservation as predicted by the modified triphasic model and the effect of natural inhomogeneities.Biophysical journal · 2012Article
- Effects of hypertonic (NaCl) two-dimensional and three-dimensional culture conditions on the properties of cartilage tissue engineered from an expanded mature bovine chondrocyte source.Tissue engineering. Part C, Methods · 2011Article
- Label-free protein profiling of adipose-derived human stem cells under hyperosmotic treatment.Journal of proteome research · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
objectiveThe increasing concentration of proteoglycans from the surface to the deep zone of articular cartilage produces a depth-dependent gradient in fixed charge density, and therefore extracellular osmolarity, which may vary with loading conditions, growth and development, or disease. In this study we examine the relationship between in situ variations in osmolarity on chondrocyte water transport properties. Chondrocytes from the depth-dependent zones of cartilage, effectively preconditioned in varying osmolarities, were used to probe this relationship.
designFirst, depth variation in osmolarity of juvenile bovine cartilage under resting and loaded conditions was characterized using a combined experimental/theoretical approach. Zonal chondrocytes were isolated into two representative "baseline" osmolarities chosen from this analysis to reflect in situ conditions. Osmotic challenge was then used as a tool for determination of water transport properties at each of these baselines. Cell calcium signaling was monitored simultaneously as a preliminary examination of osmotic baseline effects on cell signaling pathways.
resultsOsmotic baseline exhibits a significant effect on the cell membrane hydraulic permeability of certain zonal subpopulations but not on cell water content or incidence of calcium signaling.
conclusionsChondrocyte properties can be sensitive to changes in baseline osmolarity, such as those occurring during OA progression (decrease) and de novo tissue synthesis (increase). Care should be taken in comparing chondrocyte properties across zones when cells are tested in vitro in non-physiologic culture media.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.