ArticleNature protocols2009
Production of compartmented cultures of rat sympathetic neurons.
Article in Nature protocols, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed.
- Article
- Engineering a multicompartment in vitro model for dorsal root ganglia phenotypic assessment.Journal of biomedical materials research. Part B, Applied biomaterials · 2023Article
- Article
- 3D Printed Cell Culture Chamber for Testing the Effect of Pump-Based Chronic Drug Delivery on Inner Ear Tissue.Biomolecules · 2022Article
- A Brief Review of In Vitro Models for Injury and Regeneration in the Peripheral Nervous System.International journal of molecular sciences · 2022Review
- Review
- Mesenchymal stem cells and local tacrolimus delivery synergistically enhance neurite extension.Biotechnology and bioengineering · 2021Article
- Axonal mRNA translation in neurological disorders.RNA biology · 2021Review
- Active acetylcholine receptors prevent the atrophy of skeletal muscles and favor reinnervation.Nature communications · 2020Article
- Compartmentalized Neuronal Culture for Viral Transport Research.Frontiers in microbiology · 2020Review
- TTX-Resistant Sodium Channels Functionally Separate Silent From Polymodal C-nociceptors.Frontiers in cellular neuroscience · 2020Article
- c-Jun N-terminal kinase (JNK)-dependent internalization and Rab5-dependent endocytic sorting mediate long-distance retrograde neuronal death induced by axonal BDNF-p75 signaling.Scientific reports · 2019Article
- Liquid Crystal Elastomer-Based Microelectrode Array for In Vitro Neuronal Recordings.Micromachines · 2018Article
- Local NGF and GDNF levels modulate morphology and function of porcine DRG neurites, In Vitro.PloS one · 2018Article
- FAIM-L regulation of XIAP degradation modulates Synaptic Long-Term Depression and Axon Degeneration.Scientific reports · 2016Article
- Microfluidic device for the formation of optically excitable, three-dimensional, compartmentalized motor units.Science advances · 2016Article
- Tracking the Fragile X Mental Retardation Protein in a Highly Ordered Neuronal RiboNucleoParticles Population: A Link between Stalled Polyribosomes and RNA Granules.PLoS genetics · 2016Article
- Axonal spread of neuroinvasive viral infections.Trends in microbiology · 2015Review
- Assessment of TTX-s and TTX-r Action Potential Conduction along Neurites of NGF and GDNF Cultured Porcine DRG Somata.PloS one · 2015Article
- Addition of exogenous α-synuclein preformed fibrils to primary neuronal cultures to seed recruitment of endogenous α-synuclein to Lewy body and Lewy neurite-like aggregates.Nature protocols · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The compartmented culture, in which primary neurons plated in a proximal compartment send their axons under silicone grease barriers and into left and right distal compartments, has enhanced the experimental capabilities of neuronal cultures. Treatments can be applied separately to cell bodies/proximal axons or distal axons, and cell bodies/proximal axons and distal axons can be separately harvested and analyzed. Distal axons can be axotomized, and the neurons can be studied while their axons regenerate. Construction of the culture dishes requires 3 h for 48 cultures, and preparing the neurons also requires 3 h. Compartmented cultures provide enough cellular material for biochemical analyses such as immunoblotting. The uses of compartmented cultures have included studies of neurotrophic factor retrograde signaling, axonal transport, and axonal protein and lipid biosynthesis. Here we focus on sympathetic neurons cultured from neonatal rats and provide protocols for the production and some of the uses of compartmented cultures.
Indexed as
Identifiers
20010935What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.