ArticleNature protocols2009
Determination of gene expression patterns using in situ hybridization to Drosophila testes.
Article in Nature protocols, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- The Critical Impact of Sub- and Supraoptimal Temperatures on Male Fertility Potential of an Invasive Fruit Fly.Ecology and evolution · 2025Article
- Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq ofeLife · 2023Article
- The Drosophila orthologue of the primary ciliary dyskinesia-associated gene, DNAAF3, is required for axonemal dynein assembly.Biology open · 2021Article
- Drosophila SERTAD domain protein Taranis is required in somatic cells for maintenance of male germline stem cells.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Article
- iPLA2-VIA is required for healthy aging of neurons, muscle, and the female germline in Drosophila melanogaster.PloS one · 2021Article
- Cellular metabolic reprogramming controls sugar appetite in Drosophila.Nature metabolism · 2020Article
- Sperm Head-Tail Linkage Requires Restriction of Pericentriolar Material to the Proximal Centriole End.Developmental cell · 2020Article
- Tudor-domain containing protein 5-like promotes male sexual identity in the Drosophila germline and is repressed in females by Sex lethal.PLoS genetics · 2019Article
- Drosophila Pif1A is essential for spermatogenesis and is the homolog of human CCDC157, a gene associated with idiopathic NOA.Cell death & disease · 2019Article
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- cis-regulatory architecture of a short-range EGFR organizing center in the Drosophila melanogaster leg.PLoS genetics · 2018Article
- Ciliary dynein motor preassembly is regulated by Wdr92 in association with HSP90 co-chaperone, R2TP.The Journal of cell biology · 2018Article
- The importance of a halotyrosine dehalogenase forThe Journal of biological chemistry · 2018Article
- High-Quality Genome Assemblies Reveal Long Non-coding RNAs Expressed in Ant Brains.Cell reports · 2018Article
- Atrophin controls developmental signaling pathways via interactions with Trithorax-like.eLife · 2017Article
- Article
- Recruitment of Mediator Complex by Cell Type and Stage-Specific Factors Required for Tissue-Specific TAF Dependent Gene Activation in an Adult Stem Cell Lineage.PLoS genetics · 2015Article
- Relocation facilitates the acquisition of short cis-regulatory regions that drive the expression of retrogenes during spermatogenesis in Drosophila.Molecular biology and evolution · 2014Article
- Specific activation of an I-like element in Drosophila interspecific hybrids.Genome biology and evolution · 2014Article
- H3K79 methylation: a new conserved mark that accompanies H4 hyperacetylation prior to histone-to-protamine transition in Drosophila and rat.Biology open · 2014Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We describe a whole-mount RNA in situ hybridization (ISH) method optimized for detection of the cellular and subcellular distributions of specific mRNA within Drosophila testes and male genital tract. Digoxygenin (dig)-labeled antisense RNA probes are in vitro transcribed from a template synthesized by (RT)-PCR; the probe length is reduced by hydrolysis. Testes and male genital tracts are dissected from adult flies, fixed and processed for hybridization. Both probe and fixed testes can be stored before use. Extensive post-hybridization washing reduces the background. Detection is through alkaline phosphatase-conjugated anti-dig antibodies followed by a color reaction. This protocol is suitable for low-medium throughput applications with parallel processing of 2-48 samples, and takes 4-5 d to complete. We have used this protocol, which is similar to other RNA ISH protocols, but optimized for whole-mount Drosophila testes, to document the expression of about 1,000 genes in Drosophila melanogaster male genital tract.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.