ArticleAmerican journal of physiology. Endocrinology and metabolism2010
Characterization of purinergic P2X4 receptor channels expressed in anterior pituitary cells.
Article in American journal of physiology. Endocrinology and metabolism, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Aberrant alternative splicing of purinergic receptor P2RX4 prevents sensitivity towards combinatorial treatment in colorectal and pancreatic cancer.The Journal of pathology · 2026Article
- Cell-Type-Specific Expression Pattern of Proton-Sensing Receptors and Channels in Pituitary Gland.Biophysical journal · 2020Article
- Expression of P2X receptors in the rat anterior pituitary.Purinergic signalling · 2020Article
- Reduced expression of purinergic P2X4 receptors increases voluntary ethanol intake in C57BL/6J mice.Alcohol (Fayetteville, N.Y.) · 2018Article
- Neurotransmitter receptors as signaling platforms in anterior pituitary cells.Molecular and cellular endocrinology · 2018Review
- P2X4 Receptor Function in the Nervous System and Current Breakthroughs in Pharmacology.Frontiers in pharmacology · 2017Review
- Ion Channels of Pituitary Gonadotrophs and Their Roles in Signaling and Secretion.Frontiers in endocrinology · 2017Review
- Role of purinergic P2X4 receptors in regulating striatal dopamine homeostasis and dependent behaviors.Journal of neurochemistry · 2016Article
- Extracellular ATP and other nucleotides-ubiquitous triggers of intercellular messenger release.Purinergic signalling · 2016Review
- Calcium signaling properties of a thyrotroph cell line, mouse TαT1 cells.Cell calcium · 2015Article
- Purinergic signaling pathways in endocrine system.Autonomic neuroscience : basic & clinical · 2015Review
- Contribution of P2X4 receptors to ethanol intake in male C57BL/6 mice.Neurochemical research · 2014Article
- Purinergic signalling in endocrine organs.Purinergic signalling · 2014Review
- Sociocommunicative and sensorimotor impairments in male P2X4-deficient mice.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2013Article
- Pharmacological insights into the role of P2X4 receptors in behavioural regulation: lessons from ivermectin.The international journal of neuropsychopharmacology · 2013Article
- P2X receptor channels in endocrine glands.Wiley interdisciplinary reviews. Membrane transport and signaling · 2013Article
- Activation and regulation of purinergic P2X receptor channels.Pharmacological reviews · 2011Review
- Expression of P2Y receptors in the rat anterior pituitary.Purinergic signalling · 2011Article
- Allosteric modulation of ATP-gated P2X receptor channels.Reviews in the neurosciences · 2011Review
- Allosteric modulation of purine and pyrimidine receptors.Advances in pharmacology (San Diego, Calif.) · 2011Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Anterior pituitary cells express cation-conducting P2X receptor channels (P2XRs), but their molecular identity, electrophysiological properties, cell-specific expression pattern, and physiological roles have been only partially characterized. In this study, we show by quantitative RT-PCR that mRNA transcripts for the P2X(4) subunit are the most abundant in rat anterior pituitary tissue and confirm the P2X(4)R protein expression by Western blot analysis. Single-cell patch-clamp recordings show that extracellular ATP induced an inward depolarizing current in a majority of thyrotropin-releasing hormone-responsive pituitary cells, which resembled the current profile generated by recombinant P2X(4)R. The channels were activated and desensitized in a dose-dependent manner and deactivated rapidly. Activation of these channels led to stimulation of electrical activity and promotion of voltage-gated and voltage-insensitive Ca(2+) influx. In the presence of ivermectin, a specific allosteric modulator of P2X(4)Rs, there was an approximately fourfold increase in the maximum amplitude of the ATP-induced inward current, accompanied by an increase in the sensitivity of receptors for ATP, slowed deactivation of receptors, and enhanced ATP-induced prolactin release. These results indicate that thyrotropin-releasing hormone-responsive cells, including lactotrophs, express homomeric and/or heteromeric P2X(4)Rs, which facilitate Ca(2+) influx and hormone secretion.
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