ArticleThe Journal of cell biology2009
ISWI is a RanGTP-dependent MAP required for chromosome segregation.
Article in The Journal of cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 52 citations in OpenAlex.
- Subcellular localization as a driver of protein function.Nature reviews. Molecular cell biology · 2026Review
- Integrative Proteomic Analysis Reveals VMG Oncolytic Virus-Induced Molecular Reprogramming in Pancreatic Ductal Adenocarcinoma.Research square · 2026Article
- A non-transcriptional mitotic function of POU/Oct factors ensures spindle assembly and chromosome segregation.Journal of cell science · 2026Article
- Non-canonical role for the BAF complex subunit DPF3 in mitosis and ciliogenesis.Journal of cell science · 2024Article
- Unconventional roles of chromatin remodelers and long non-coding RNAs in cell division.Cellular and molecular life sciences : CMLS · 2023Review
- The large cytoplasmic volume of oocyte.The Journal of reproduction and development · 2023Review
- Molecular Link between DNA Damage Response and Microtubule Dynamics.International journal of molecular sciences · 2022Review
- The ATPase SRCAP is associated with the mitotic apparatus, uncovering novel molecular aspects of Floating-Harbor syndrome.BMC biology · 2021Article
- Moonlighting in Mitosis: Analysis of the Mitotic Functions of Transcription and Splicing Factors.Cells · 2020Review
- CCDC74A/B are K-fiber crosslinkers required for chromosomal alignment.BMC biology · 2019Article
- Chromosome alignment maintenance requires the MAP RECQL4, mutated in the Rothmund-Thomson syndrome.Life science alliance · 2019Article
- Mitotic Gene Bookmarking: An Epigenetic Program to Maintain Normal and Cancer Phenotypes.Molecular cancer research : MCR · 2018Review
- Review
- Article
- Genome maintenance functions of the INO80 chromatin remodeller.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Developmentally Regulated GTP binding protein 1 (DRG1) controls microtubule dynamics.Scientific reports · 2017Article
- Mechanisms of Mitotic Spindle Assembly.Annual review of biochemistry · 2016Review
- Identification of the ISWI Chromatin Remodeling Complex of the Early Branching Eukaryote Trypanosoma brucei.The Journal of biological chemistry · 2015Article
- An epigenetic regulator emerges as microtubule minus-end binding and stabilizing factor in mitosis.Nature communications · 2015Article
- Nuclear transport factors: global regulation of mitosis.Current opinion in cell biology · 2015Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Production of RanGTP around chromosomes induces spindle assembly by activating nuclear localization signal (NLS)-containing factors. Here, we show that the NLS protein ISWI, a known chromatin-remodeling ATPase, is a RanGTP-dependent microtubule (MT)-associated protein. Recombinant ISWI induces MT nucleation, stabilization, and bundling in vitro. In Xenopus culture cells and egg extract, ISWI localizes within the nucleus in interphase and on spindles during mitosis. Depletion of ISWI in egg extracts does not affect spindle assembly, but in anaphase spindle MTs disappear and chromosomes do not segregate. We show directly that ISWI is required for the RanGTP-dependent stabilization of MTs during anaphase independently of its effect on chromosomes. ISWI depletion in Drosophila S2 cells induces defects in spindle MTs and chromosome segregation in anaphase, and the cells eventually stop growing. Our results demonstrate that distinctly from its role in spindle assembly, RanGTP maintains spindle MTs in anaphase through the local activation of ISWI and that this is essential for proper chromosome segregation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.