ArticleNucleic acids research2010
Imprecise transcription termination within Escherichia coli greA leader gives rise to an array of short transcripts, GraL.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A simple and unified protocol to purify all seven Escherichia coli RNA polymerase sigma factors.Journal of applied genetics · 2024Article
- A Holling Functional Response Model for Mapping QTLs Governing Interspecific Interactions.Frontiers in genetics · 2021Article
- Interactions between DksA and Stress-Responsive Alternative Sigma Factors Control Inorganic Polyphosphate Accumulation in Escherichia coli.Journal of bacteriology · 2020Article
- Autoregulation ofInternational journal of molecular sciences · 2019Article
- A mapping framework of competition-cooperation QTLs that drive community dynamics.Nature communications · 2018Article
- Unprecedented high-resolution view of bacterial operon architecture revealed by RNA sequencing.mBio · 2014Article
- Finding consensus stable local optimal structures for aligned RNA sequences and its application to discovering riboswitch elements.International journal of bioinformatics research and applications · 2014Article
- Complex transcriptional and post-transcriptional regulation of an enzyme for lipopolysaccharide modification.Molecular microbiology · 2013Article
- Effects on growth by changes of the balance between GreA, GreB, and DksA suggest mutual competition and functional redundancy in Escherichia coli.Journal of bacteriology · 2012Article
- RNA folding in transcription elongation complex: implication for transcription termination.The Journal of biological chemistry · 2011Article
- Circuitry linking the Csr and stringent response global regulatory systems.Molecular microbiology · 2011Article
- Premature terminator analysis sheds light on a hidden world of bacterial transcriptional attenuation.Genome biology · 2010Article
- Transcription termination by the eukaryotic RNA polymerase III.Biochimica et biophysica actaReview
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Authors and funding
5 authors.
Funding
Abstract
We report that greA expression is driven by two strong, overlapping P1 and P2 promoters. The P1 promoter is sigma(70)-dependent and P2 is sigma(E)-dependent. Two-thirds of transcripts terminate within the leader region and the remaining third comprises greA mRNA. Termination efficiency seems to be unaffected by growth phase. Two collections of small 40-50 (initiating from P2) and 50-60 nt (from P1) RNA chains, termed GraL, are demonstrable in vivo and in vitro. We document that GraL arrays arise from an intrinsic terminator with an 11 bp stem followed by an AU(7)GCU(2) sequence. Atypical chain termination occurs at multiple sites; the 3'-ends differ by 1 nt over a range of 10 nt. Transcripts observed are shown to be insensitive to Gre factors and physically released from RNAP-DNA complexes. The abundance of individual chains within each cluster displays a characteristic pattern, which can be differentially altered by oligonucleotide probes. Multiple termination sites are particularly sensitive to changes at the bottom of the stem. Evolutionarily conserved GraL stem structures and fitness assays suggest a biological function for the RNA clusters themselves. Although GraL overexpression induces >/=3-fold transcriptional changes of over 100 genes, a direct target remains elusive.
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