ArticleJournal of bacteriology2010
Temporal and spatial regulation of the four transcription start sites of hetR from Anabaena sp. strain PCC 7120.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 40 citations in OpenAlex.
- R-DeeP/TripepSVM identifies the RNA-binding OB-fold-like protein PatR as regulator of heterocyst patterning.Nucleic acids research · 2025Article
- mRNA localization and thylakoid protein biogenesis in the filamentous heterocyst-forming cyanobacteriumJournal of bacteriology · 2024Article
- Terminal heterocyst differentiation in the Anabaena patA mutant as a result of post-transcriptional modifications and molecular leakage.PLoS computational biology · 2022Article
- Interaction network among factors involved in heterocyst-patterning in cyanobacteria.Molecular genetics and genomics : MGG · 2022Article
- The Heterocyst-Specific Small RNA NsiR1 Regulates the Commitment to Differentiation in Nostoc.Microbiology spectrum · 2022Article
- Mathematical models of nitrogen-fixing cell patterns in filamentous cyanobacteria.Frontiers in cell and developmental biology · 2022Review
- Inactivation of Three RG(S/T)GR Pentapeptide-Containing Negative Regulators of HetR Results in Lethal Differentiation ofLife (Basel, Switzerland) · 2020Article
- HetL, HetR and PatS form a reaction-diffusion system to control pattern formation in the cyanobacteriumeLife · 2020Article
- Expression from DIF1-motif promoters of hetR and patS is dependent on HetZ and modulated by PatU3 during heterocyst differentiation.PloS one · 2020Article
- Small RNAs Involved in Regulation of Nitrogen Metabolism.Microbiology spectrum · 2018Review
- Formation and maintenance of nitrogen-fixing cell patterns in filamentous cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Regulatory RNAs in photosynthetic cyanobacteria.FEMS microbiology reviews · 2015Review
- Comparative analysis of the primary transcriptome of Synechocystis sp. PCC 6803.DNA research : an international journal for rapid publication of reports on genes and genomes · 2014Article
- Article
- Article
- Identification of the HetR recognition sequence upstream of hetZ in Anabaena sp. strain PCC 7120.Journal of bacteriology · 2012Article
- Dynamics of transcriptional start site selection during nitrogen stress-induced cell differentiation in Anabaena sp. PCC7120.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Structure of transcription factor HetR required for heterocyst differentiation in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Transcriptional regulation of the heterocyst patterning gene patA from Anabaena sp. strain PCC 7120.Journal of bacteriology · 2010Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The filamentous cyanobacterium Anabaena sp. strain PCC 7120 forms nitrogen-fixing heterocysts in a periodic pattern in response to combined-nitrogen limitation in the environment. The master regulator of heterocyst differentiation, HetR, is necessary for both pattern formation and commitment of approximately every 10th cell of a filament to differentiation into a heterocyst. In this study, the individual contributions of four transcriptional start points (tsps) in regulation of transcription of hetR were assessed, and the effects of the regulatory genes patS, hetN, and patA on transcription from the tsps were determined. The tsp located at nucleotide -271 relative to the translational start site (-271 tsp) was the most tightly regulated tsp, and fluorescence from a -271 tsp-green fluorescent protein (GFP) reporter fusion was observed initially in groups of two cells and later in single cells arranged in a spatial pattern that mimicked the pattern of heterocysts that emerged. Conversely, the fluorescence from the -184 and -728/-696 tsp-GFP reporter fusions was uniform throughout filaments. Transcription from the -271 tsp was severely downregulated in a strain in which the patA gene, which encodes a positive regulator of differentiation, was deleted, and it was not detectable in strains overexpressing the genes encoding the negative regulators of differentiation, patS and hetN. In strains lacking the -271 tsp of hetR, pattern formation, the timing of commitment to differentiation, and the number of cells that differentiated into heterocysts were affected. Taken together, these results demonstrate the role of regulation of the -271 tsp of hetR in the genetic network that governs heterocyst pattern formation and differentiation.
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