ArticleJournal of bacteriology2010
Genetic analysis of the regulation of type IV pilus function by the Chp chemosensory system of Pseudomonas aeruginosa.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
89 citing papers in PubMed, 152 citations in OpenAlex.
- Bacteria regulate collective behaviour by mechanosensing cell-cell collisions.Nature microbiology · 2026Article
- Distinct roles of PilI and ChpC inApplied and environmental microbiology · 2026Article
- Tandem receiver domain response regulator DrdR modulates swarming by controlling PilB/PilT activity inmLife · 2026Article
- Enhanced identification of key bacterial motility genes via a cross-species genomic hybrid feature machine learning approach.BMC genomics · 2026Article
- Contribution of two-component regulatory systems to the acute-to-chronic infection transition ofJournal of bacteriology · 2026Review
- Type IV pilin regulation: a transcriptional overview.Critical reviews in microbiology · 2026Review
- Proteomic Analysis of the Differential Response ofAntibiotics (Basel, Switzerland) · 2025Article
- The type IV pilus steering committee: how Pil-Chp controls directional motility.Journal of bacteriology · 2025Review
- Achromobacter xylosoxidans modulates Pseudomonas aeruginosa virulence through a complex multi-target competition.Scientific reports · 2025Article
- Antagonistic response regulators spatially regulate receptor methylation in the Pseudomonas aeruginosa Pil-Chp surface sensing system.Cell reports · 2025Article
- Hyperpiliation, not loss of pilus retraction, reducesMicrobiology spectrum · 2025Article
- Xanthomonas oryzae Orphan Response Regulator EmvR Is Involved in Virulence, Extracellular Polysaccharide Production and Cell Motility.Molecular plant pathology · 2025Article
- A Phage-Based Approach to Identify Antivirulence Inhibitors of Bacterial Type IV Pili.Microbial biotechnology · 2025Article
- Evidence of universal conformal invariance in living biological matter.Nature physics · 2025Article
- Structure of the Pseudomonas aeruginosa PAO1 Type IV pilus.PLoS pathogens · 2024Article
- Interspecies surfactants serve as public goods enabling surface motility inJournal of bacteriology · 2024Article
- The surface interface and swimming motility influence surface-sensing responses inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Individual bacterial cells can use spatial sensing of chemical gradients to direct chemotaxis on surfaces.Nature microbiology · 2024Article
- A bacterial sense of touch: T4P retraction motor as a means of surface sensing byJournal of bacteriology · 2024Review
- Review
29 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The virulence of the opportunistic pathogen Pseudomonas aeruginosa involves the coordinate expression of many virulence factors, including type IV pili, which are required for colonization of host tissues and for twitching motility. Type IV pilus function is controlled in part by the Chp chemosensory system, which includes a histidine kinase, ChpA, and two CheY-like response regulators, PilG and PilH. How the Chp components interface with the type IV pilus motor proteins PilB, PilT, and PilU is unknown. We present genetic evidence confirming the role of ChpA, PilG, and PilB in the regulation of pilus extension and the role of PilH and PilT in regulating pilus retraction. Using informative double and triple mutants, we show that (i) ChpA, PilG, and PilB function upstream of PilH, PilT, and PilU; (ii) that PilH enhances PilT function; and (iii) that PilT and PilB retain some activity in the absence of signaling input from components of the Chp system. By site-directed mutagenesis, we demonstrate that the histidine kinase domain of ChpA and the phosphoacceptor sites of both PilG and PilH are required for type IV pilus function, suggesting that they form a phosphorelay system important in the regulation of pilus extension and retraction. Finally, we present evidence suggesting that pilA transcription is regulated by intracellular PilA levels. We show that PilA is a negative regulator of pilA transcription in P. aeruginosa and that the Chp system functionally regulates pilA transcription by controlling PilA import and export.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.