ArticleThe Journal of molecular diagnostics : JMD2010
High-throughput identification and quantification of Candida species using high resolution derivative melt analysis of panfungal amplicons.
Article in The Journal of molecular diagnostics : JMD, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 54 citations in OpenAlex.
- New strategy for rapid diagnosis and characterization of fungal infections: the example of corneal scrapings.PloS one · 2012Trial
- Cross-kingdom pathogen detection via duplex universal PCR and high-resolution melt.Biosensors & bioelectronics · 2025Article
- Investigation of adhesion status of Candida species to the surface of resin materials produced at different angles with additive manufacturing.BMC oral health · 2024Article
- Development of a high-resolution melt-based assay to rapidly detect the azole-resistantCurrent medical mycology · 2023Article
- Direct detection of Corynebacterium striatum, Corynebacterium propinquum, and Corynebacterium simulans in sputum samples by high-resolution melt curve analysis.BMC infectious diseases · 2021Article
- Rapid detection of ERG11 polymorphism associated azole resistance in Candida tropicalis.PloS one · 2021Article
- Efficient differentiation ofJournal of medical microbiology · 2020Article
- Molecular identification of Candida isolates by Real-time PCR-high-resolution melting analysis and investigation of the genetic diversity of Candida species.Journal of clinical laboratory analysis · 2020Article
- Rapid identification of the Candida glabrata species complex by high-resolution melting curve analysis.Journal of clinical laboratory analysis · 2020Article
- A forest-specialist carnivore in the middle of the desert?Comments on https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.5230.Ecology and evolution · 2020Article
- Recent trends in molecular diagnostics of yeast infections: from PCR to NGS.FEMS microbiology reviews · 2019Review
- Speciation of fungi using real time PCR with molecular beacons: Can we solve the enigma of diagnosis of invasive fungal disease?Medical journal, Armed Forces India · 2019Article
- Prevalence of candidemia and associated candida subtypes following severe sepsis in non-neutropenic critically ill patients.Acta bio-medica : Atenei Parmensis · 2018Article
- Microbial Typing by Machine Learned DNA Melt Signatures.Scientific reports · 2017Article
- Dynamic time warping assessment of high-resolution melt curves provides a robust metric for fungal identification.PloS one · 2017Article
- Principles of a New Protocol for Prediction of Azole Resistance in Candida albicans Infections on the Basis of ERG11 Polymorphisms.Current microbiology · 2016Article
- Development of Candida-Specific Real-Time PCR Assays for the Detection and Identification of Eight Medically Important Candida Species.Microbiology insights · 2016Article
- The Role of PCR in the Diagnosis of Candida Vulvovaginitis-a New Gold Standard?Current infectious disease reports · 2015Article
- Multicenter evaluation of Candida QuickFISH BC for identification of Candida species directly from blood culture bottles.Journal of clinical microbiology · 2015Article
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Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Fungal infections pose unique challenges to molecular diagnostics; fungal molecular diagnostics consequently lags behind bacterial and viral counterparts. Nevertheless, fungal infections are often life-threatening, and early detection and identification of species is crucial to successful intervention. A high throughput PCR-based method is needed that is independent of culture, is sensitive to the level of one fungal cell per milliliter of blood or other tissue types, and is capable of detecting species and resistance mutations. We introduce the use of high resolution melt analysis, in combination with more sensitive, inclusive, and appropriately positioned panfungal primers, to address these needs. PCR-based amplification of the variable internal transcribed regions of the rDNA genes generates an amplicon whose sequence melts with a shape that is characteristic and therefore diagnostic of the species. Simple analysis of the differences between test and reference melt curves generates a single number that calls the species. Early indications suggest that high resolution melt analysis can distinguish all eight major species of Candida of clinical significance without interference from excess human DNA. Candida species, including mixed and novel species, can be identified directly in vaginal samples. This tool can potentially detect, count, and identify fungi in hundreds of samples per day without further manipulation, costs, or delays, offering a major step forward in fungal molecular diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.