ArticleInvestigative ophthalmology & visual science2010
Epifluorescence intravital microscopy of murine corneal dendritic cells.
Article in Investigative ophthalmology & visual science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 56 citations in OpenAlex.
- CD4+CD25- T-Cell-Secreted IFN-γ Promotes Corneal Nerve Degeneration in Diabetic Mice.Investigative ophthalmology & visual science · 2025Article
- Corneal nerve abnormalities in early-stage diabetic retinopathy evaluated by corneal confocal microscopy.BMC ophthalmology · 2025Article
- Increased dendritic cell density and altered morphology in allergic conjunctivitis.Eye (London, England) · 2023Observational
- The miR-183/96/182 cluster is a checkpoint for resident immune cells and shapes the cellular landscape of the cornea.The ocular surface · 2023Article
- Dendritiform immune cells with reduced antigen-capture capacity persist in the cornea during the asymptomatic phase of allergic conjunctivitis.Eye (London, England) · 2023Article
- Tissue-like environments shape functional interactions of HIV-1 with immature dendritic cells.EMBO reports · 2023Article
- Diurnal changes and topographical distribution of ocular surface epithelial dendritic cells in humans, and repeatability of density and morphology assessment.Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians (Optometrists) · 2023Article
- Laser-induced microinjury of the corneal basal epithelium and imaging of resident macrophage responses in a live, whole-eye preparation.Frontiers in immunology · 2023Article
- Corneal dendritic cells in diabetes mellitus: A narrative review.Frontiers in endocrinology · 2023Review
- The impact of sensory neuropathy and inflammation on epithelial wound healing in diabetic corneas.Progress in retinal and eye research · 2022Review
- Macrophage recruitment in immune-privileged lens during capsule repair, necrotic fiber removal, and fibrosis.iScience · 2021Article
- Corneal Immune Cells Are Increased in Patients With Multiple Sclerosis.Translational vision science & technology · 2021Article
- Altered Corneal Epithelial Dendritic Cell Morphology and Phenotype Following Acute Exposure to Hyperosmolar Saline.Investigative ophthalmology & visual science · 2021Article
- The miR-183/96/182 Cluster Regulates the Functions of Corneal Resident Macrophages.ImmunoHorizons · 2020Article
- In vivo immune cell dynamics in the human cornea.Experimental eye research · 2020Observational
- The neuroregenerative effects of topical decorin on the injured mouse cornea.Journal of neuroinflammation · 2020Article
- Kinetics of corneal leukocytes by intravital multiphoton microscopy.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Induction of Immune Surveillance of the Dysmorphogenic Lens.Scientific reports · 2017Article
- Review
- IL-20 promotes epithelial healing of the injured mouse cornea.Experimental eye research · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Purpose. Dendritic cells (DCs) are antigen-presenting cells vital for initiating immune responses. In this study the authors examined the in vivo migratory capability of resident corneal DCs to various stimuli. Methods. The authors used mice expressing enhanced yellow fluorescent protein (eYFP) under control of the CD11c promoter to visualize corneal DCs. To assess the distribution and mobility of DCs, normal corneas were imaged in vivo and ex vivo with fluorescence microscopy. Intravital microscopy was used to examine the responses of resident central and peripheral corneal DCs to silver nitrate injury, lipopolysaccharide, microspheres, and tumor necrosis factor (TNF-alpha). In some experiments, TNF-alpha injection was used to first induce centripetal migration of DCs to the central cornea, which was subsequently reinjected with microspheres. Results. In normal corneas, DCs were sparsely distributed centrally and were denser in the periphery, with epithelial-level DCs extending into the epithelium. Videomicroscopy showed that though cell processes were in continuous movement, cells generally did not migrate. Within the first 6 hours after stimulation, neither central nor peripheral corneal DCs exhibited significant lateral migration, but central corneal DCs assumed extreme morphologic changes. An increased number of DCs in the TNF-alpha-stimulated central cornea were responsive to subsequent microsphere injection by adopting a migratory behavior, but not with increased speed. Conclusions. In vivo imaging reveals minimal lateral migration of corneal DCs after various stimuli. In contrast, DCs within the central cornea after initial TNF-alpha injection are more likely to respond to a secondary insult with lateral migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.