ArticleProceedings of the National Academy of Sciences of the United States of America2009
FM dyes enter via a store-operated calcium channel and modify calcium signaling of cultured astrocytes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- PHluorin-conjugated secondary nanobodies as a tool for measuring synaptic vesicle exocytosis and endocytosis.Scientific reports · 2025Article
- Article
- Charge and lipophilicity are required for effective block of the hair-cell mechano-electrical transducer channel by FM1-43 and its derivatives.Frontiers in cell and developmental biology · 2023Article
- Exocytosis in Astrocytes.Biomolecules · 2021Review
- Exocytosis of large-diameter lysosomes mediates interferon γ-induced relocation of MHC class II molecules toward the surface of astrocytes.Cellular and molecular life sciences : CMLS · 2020Article
- Embryonic Stem Cell-Derived Peripheral Auditory Neurons Form Neural Connections with Mouse Central Auditory Neurons In Vitro via the α2δ1 Receptor.Stem cell reports · 2018Article
- Stretch-induced CaThe Journal of physiology · 2018Article
- Multiple Mechanisms Drive Calcium Signal Dynamics around Laser-Induced Epithelial Wounds.Biophysical journal · 2017Article
- Exploring cells with targeted biosensors.The Journal of general physiology · 2017Review
- Astrocyte VAMP3 vesicles undergo Ca2+ -independent cycling and modulate glutamate transporter trafficking.The Journal of physiology · 2015Article
- A novel size-based sorting mechanism of pinocytic luminal cargoes in microglia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Examination of synaptic vesicle recycling using FM dyes during evoked, spontaneous, and miniature synaptic activities.Journal of visualized experiments : JoVE · 2014Article
- Eliminating unwanted far-field excitation in objective-type TIRF. Part II. combined evanescent-wave excitation and supercritical-angle fluorescence detection improves optical sectioning.Biophysical journal · 2014Article
- Eliminating unwanted far-field excitation in objective-type TIRF. Part I. identifying sources of nonevanescent excitation light.Biophysical journal · 2014Article
- Abnormal intracellular calcium signaling and SNARE-dependent exocytosis contributes to SOD1G93A astrocyte-mediated toxicity in amyotrophic lateral sclerosis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- A critical role for STIM1 in filopodial calcium entry and axon guidance.Molecular brain · 2013Article
- New tools for investigating astrocyte-to-neuron communication.Frontiers in cellular neuroscience · 2013Review
- Fluorescent probes for monitoring regulated secretion.Current opinion in chemical biology · 2013Review
- APP+, a fluorescent analogue of the neurotoxin MPP+, is a marker of catecholamine neurons in brain tissue, but not a fluorescent false neurotransmitter.ACS chemical neuroscience · 2013Article
- Direct imaging of ER calcium with targeted-esterase induced dye loading (TED).Journal of visualized experiments : JoVE · 2013Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The amphiphilic fluorescent styryl pyridinium dyes FM1-43 and FM4-64 are used to probe activity-dependent synaptic vesicle cycling in neurons. Cultured astrocytes can internalize FM1-43 and FM4-64 inside vesicles but their uptake is insensitive to the elevation of cytosolic calcium (Ca(2+)) concentration and the underlying mechanism remains unclear. Here we used total internal reflection fluorescence microscopy and pharmacological tools to study the mechanisms of FM4-64 uptake into cultured astrocytes from mouse neocortex. Our data show that: (i) endocytosis is not a major route for FM4-64 uptake into astrocytes; (ii) FM4-64 enters astrocytes through an aqueous pore and strongly affects Ca(2+) homeostasis; (iii) partitioning of FM4-64 into the outer leaflet of the plasma membrane results in a facilitation of store-operated Ca(2+) entry (SOCE) channel gating; (iv) FM4-64 permeates and competes with Ca(2+) for entry through a SOCE channel; (v) intracellular FM4-64 mobilizes Ca(2+) from the endoplasmic reticulum stores, conveying a positive feedback to activate SOCE and to sustain dye uptake into astrocytes. Our study demonstrates that FM dyes are not markers of cycling vesicles in astrocytes and calls for a careful interpretation of FM fluorescence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.