ArticleJournal of experimental botany2010
Silymarin secretion and its elicitation by methyl jasmonate in cell cultures of Silybum marianum is mediated by phospholipase D-phosphatidic acid.
Article in Journal of experimental botany, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Phospholipid Signaling Is a Component of the Salicylic Acid Response in Plant Cell Suspension Cultures.International journal of molecular sciences · 2020Review
- Enhancement of silymarin and phenolic compound accumulation in tissue culture of Milk thistle using elicitor feeding and hairy root cultures.Journal, genetic engineering & biotechnology · 2016Article
- A Phosphatidic Acid (PA) conveyor system of continuous intracellular transport from cell membrane to nucleus maintains EGF receptor homeostasis.Oncotarget · 2016Article
- Salicylic acid induces vanillin synthesis through the phospholipid signaling pathway in Capsicum chinense cell cultures.Plant signaling & behavior · 2013Article
- Phosphoglycerolipids are master players in plant hormone signal transduction.Plant cell reports · 2013Review
- Involvement of phospholipases C and D in the defence responses of riboflavin-treated tobacco cells.Protoplasma · 2013Article
- Improved silymarin content in elicited multiple shoot cultures of Silybum marianum L.Physiology and molecular biology of plants : an international journal of functional plant biology · 2013Article
- Phytophthora infestans has a plethora of phospholipase D enzymes including a subclass that has extracellular activity.PloS one · 2011Article
Corrections and comments
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Authors and funding
2 authors.
Funding
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Abstract
The flavonolignan silymarin is released to the extracellular medium of Silybum marianum cultures and its production can be stimulated by the elicitor methyljasmonate (MeJA). The sequence of the signalling processes leading to this response is unknown at present. It is reported in this work that MeJA increased the activity of the enzyme phospholipase D (PLD). Treatment with mastoparan (Mst), a PLD activity stimulator, also enhanced PLD and caused a substantial increase in silymarin production. The application of the product of PLD activity, phosphatidic acid (PA) promoted silymarin accumulation. Altering PLD activity by introducing in cultures n-butanol (nBuOH), which inhibits PA production by PLD, prevented silymarin elicitation by MeJA or Mst and also impeded its release in non-elicited cultures. Treatment with iso-, sec- or tert- butanol had no effect on silymarin production. The exogenous addition of PA reversed the inhibitory action of nBuOH, both in control and MeJA-treated cultures. These results suggest that the enzyme PLD and its product PA mediate silymarin secretion to the medium of S. marianum cultures.
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