ArticleBiophysical journal2009
Quick shear-flow alignment of biological filaments for X-ray fiber diffraction facilitated by methylcellulose.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Dissecting Structural and Functional Determinants of Microtubules Stabilization through Guided Chemical Modulation.Journal of medicinal chemistry · 2026Article
- Spatiotemporal Control of Formation of Dynamic Protein Fiber Assemblies via Photophysical Effects of a Focused Laser Beam.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Shear-Induced Anisotropic Supramolecular Gel Noodles for Improved Cell Guidance in Polarized Tissue Engineering.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- X-ray diffraction recording from a small amount of fibrous protein materials oriented by a micro shear-flow cell.Biophysics and physicobiology · 2024Article
- Review
- Fiber Diffraction and Small-Angle Scattering for Structural Investigation of Bacterial Amyloids.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- Taxanes convert regions of perturbed microtubule growth into rescue sites.Nature materials · 2020Article
- X-Ray Fiber Diffraction Recordings from Oriented Demembranated Chlamydomonas Flagellar Axonemes.Biophysical journal · 2015Article
- Hard X-ray Fourier transform holography from an array of oriented referenced objects.Journal of synchrotron radiation · 2011Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
X-ray fiber diffraction is one of the most useful methods for examining the structural details of live biological filaments under physiological conditions. To investigate biologically active or labile materials, it is crucial to finish fiber alignment within seconds before diffraction analysis. However, the conventional methods, e.g., magnetic field alignment and low-speed centrifugations, are time-consuming and not very useful for such purposes. Here, we introduce a new alignment method using a rheometer with two parallel disks, which was applied to observe fiber diffractions of axonemes, tobacco mosaic tobamovirus, and microtubules. We found that fibers were aligned within 5 s by giving high shear flow (1000-5000 s(-1)) to the medium and that methylcellulose contained in the medium (approximately 1%) was essential to the accomplishment of uniform orientation with a small angular deviation (<5 degrees). The new alignment method enabled us to execute structure analyses of axonemes by small-angle x-ray diffraction. Since this method was also useful for the quick alignment of purified microtubules, as well as tobacco mosaic tobamovirus, we expect that we can apply it to the structural analysis of many other biological filaments.
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