ArticleBiophysical journal2009
Combining laser microsurgery and finite element modeling to assess cell-level epithelial mechanics.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed.
- Review
- The mechanopathology of the tumor microenvironment: detection techniques, molecular mechanisms and therapeutic opportunities.Frontiers in cell and developmental biology · 2025Review
- Direct force measurement and loading on developing tissues in intact avian embryos.Development (Cambridge, England) · 2023Article
- Homeotic compartment curvature and tension control spatiotemporal folding dynamics.Nature communications · 2023Article
- Generation, Transmission, and Regulation of Mechanical Forces in Embryonic Morphogenesis.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- Two-Photon Cell and Tissue Level Laser Ablation Methods to Study Morphogenetic Biomechanics.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Cell-substrate mechanics guide collective cell migration through intercellular adhesion: a dynamic finite element cellular model.Biomechanics and modeling in mechanobiology · 2020Article
- Measuring mechanical stress in living tissues.Nature reviews. Physics · 2020Article
- Viscoelastic voyages - Biophysical perspectives on cell intercalation during Drosophila gastrulation.Seminars in cell & developmental biology · 2020Review
- Biophysical principles of choanoflagellate self-organization.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Cell rearrangement induced by filopodial tension accounts for the late phase of convergent extension in the sea urchin archenteron.Molecular biology of the cell · 2019Article
- Elongated Cells Drive Morphogenesis in a Surface-Wrapped Finite-Element Model of Germband Retraction.Biophysical journal · 2019Article
- Dynamics of PAR Proteins Explain the Oscillation and Ratcheting Mechanisms in Dorsal Closure.Biophysical journal · 2018Article
- Quantifying forces in cell biology.Nature cell biology · 2017Review
- Inferring cellular forces from image stacks.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Article
- Dynamic cellular finite-element method for modelling large-scale cell migration and proliferation under the control of mechanical and biochemical cues: a study of re-epithelialization.Journal of the Royal Society, Interface · 2017Article
- Non-straight cell edges are important to invasion and engulfment as demonstrated by cell mechanics model.Biomechanics and modeling in mechanobiology · 2016Article
- Pathway to a phenocopy: Heat stress effects in early embryogenesis.Developmental dynamics : an official publication of the American Association of Anatomists · 2016Article
- Direct laser manipulation reveals the mechanics of cell contacts in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Practical aspects of the cellular force inference toolkit (CellFIT).Methods in cell biology · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laser microsurgery and finite element modeling are used to determine the cell-level mechanics of the amnioserosa-a morphogenetically crucial epithelium on the dorsal surface of fruit fly embryos (Drosophila melanogaster). In the experiments, a tightly focused laser ablates a subcellular hole (1 microm in diameter) that passes clean through the epithelium. The surrounding cells recoil from the wound site with a large range of initial recoil velocities. These depend on the embryo's developmental stage and the subcellular wound site. The initial recoil (up to 0.1 s) is well reproduced by a base finite element model, which assumes a uniform effective viscosity inside the cells, a constant tension along each cell-cell boundary, and a large, potentially anisotropic, far-field stress--one that far exceeds the stress equivalent of the cell-edge tensions. After 0.1 s, the experimental recoils slow dramatically. This observation can be reproduced by adding viscoelastic rods along cell edges or as a fine prestressed mesh parallel to the apical and basal membranes of the cell. The mesh also reproduces a number of double-wounding experiments in which successive holes are drilled in a single cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.