ArticleJournal of molecular biology2010
The prototypical H+/galactose symporter GalP assembles into functional trimers.
Article in Journal of molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 30 citations in OpenAlex.
- Integrated laboratory evolution and rational engineering of GalP/Glk-dependentMetabolic engineering communications · 2021Article
- Sample Preparation and Data Collection for Electron Crystallographic Studies on Membrane Protein Structures and Lipid-Protein Interaction.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Protein-Protein Interactions in the Cytoplasmic Membrane of Escherichia coli: Influence of the Overexpression of Diverse Transporter-Encoding Genes on the Activities of PTS Sugar Uptake Systems.Microbial physiology · 2020Article
- DNA-Corralled Nanodiscs for the Structural and Functional Characterization of Membrane Proteins and Viral Entry.Journal of the American Chemical Society · 2018Article
- Sparse and incomplete factorial matrices to screen membrane protein 2D crystallization.Journal of structural biology · 2015Article
- Molecular Mechanism Underlying the Plant NRT1.1 Dual-Affinity Nitrate Transporter.Frontiers in physiology · 2015Review
- The collection of high-resolution electron diffraction data.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Advances in structural and functional analysis of membrane proteins by electron crystallography.Structure (London, England : 1993) · 2011Review
- Structural underpinnings of nitrogen regulation by the prototypical nitrogen-responsive transcriptional factor NrpR.Structure (London, England : 1993) · 2010Article
- The binding of cholera toxin to the periplasmic vestibule of the type II secretion channel.Channels (Austin, Tex.)Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Glucose is a primary source of energy for human cells. Glucose transporters form specialized membrane channels for the transport of sugars into and out of cells. Galactose permease (GalP) is the closest bacterial homolog of human facilitated glucose transporters. Here, we report the functional reconstitution and 2D crystallization of GalP. Single particle electron microscopy analysis of purified GalP shows that the protein assembles as an oligomer with three distinct densities. Reconstitution assays yield 2D GalP crystals that exhibit a hexagonal array having p3 symmetry. The projection structure of GalP at 18 A resolution shows that the protein is trimeric. Each monomer in the trimer forms its own channel, but an additional cavity (10 approximately 15 A in diameter) is apparent at the 3-fold axis of the oligomer. We show that the crystalline GalP is able to selectively bind substrate, suggesting that the trimeric form is biologically active.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.