ArticleBMC plant biology2009
A high-density collection of EMS-induced mutations for TILLING in Landsberg erecta genetic background of Arabidopsis.
Article in BMC plant biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 73 citations in OpenAlex.
- Ethyl methanesulfonate mutagenesis in fungi: genetic mechanisms, applications, and implications for agricultural biotechnology.Archives of microbiology · 2026Review
- Forward genetic screening of mutants of the wild diploid ancestor Nicotiana sylvestris identifies aspartate oxidase 2 as a key gene for nicotine biosynthesis in cultivated allotetraploid tobacco (Nicotiana tabacum).The Plant journal : for cell and molecular biology · 2026Article
- Variations in Total Protein and Amino Acids in the Sequenced Sorghum Mutant Library.Plants (Basel, Switzerland) · 2023Article
- Augmenting tomato functional genomics with a genome-wide induced genetic variation resource.Frontiers in plant science · 2023Article
- Genome-wide analysis of mutations induced by carbon ion beam irradiation in cotton.Frontiers in plant science · 2023Article
- Ultra-high static magnetic field induces a change in the spectrum but not frequency of DNA spontaneous mutations inFrontiers in plant science · 2023Article
- Construction and analysis of a Noccaea caerulescens TILLING population.BMC plant biology · 2022Article
- Article
- Genome-Wide Analysis of Artificial Mutations Induced by Ethyl Methanesulfonate in the Eggplant (Genes · 2019Article
- Generation and Characterization of a Foxtail Millet (Frontiers in plant science · 2019Article
- The HEM Lines: A New Library of HomozygousFrontiers in plant science · 2018Article
- The Analysis of Physiological Variations in MFrontiers in plant science · 2017Article
- Elucidating the role of highly homologousPlant methods · 2017Article
- First TILLING platform in Cucurbita pepo: a new mutant resource for gene function and crop improvement.PloS one · 2014Article
- PT-Flax (phenotyping and TILLinG of flax): development of a flax (Linum usitatissimum L.) mutant population and TILLinG platform for forward and reverse genetics.BMC plant biology · 2013Article
- Production of a high-efficiency TILLING population through polyploidization.Plant physiology · 2013Article
- A mutant Brassica napus (canola) population for the identification of new genetic diversity via TILLING and next generation sequencing.PloS one · 2013Article
- A diploid wheat TILLING resource for wheat functional genomics.BMC plant biology · 2012Article
- High-throughput discovery of mutations in tef semi-dwarfing genes by next-generation sequencing analysis.Genetics · 2012Article
- Genome-wide analysis of mutations in mutant lineages selected following fast-neutron irradiation mutagenesis of Arabidopsis thaliana.Genome research · 2012Article
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4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundArabidopsis thaliana is the main model species for plant molecular genetics studies and world-wide efforts are devoted to identify the function of all its genes. To this end, reverse genetics by TILLING (Targeting Induced Local Lesions IN Genomes) in a permanent collection of chemically induced mutants is providing a unique resource in Columbia genetic background. In this work, we aim to extend TILLING resources available in A. thaliana by developing a new population of ethyl methanesulphonate (EMS) induced mutants in the second commonest reference strain. In addition, we pursue to saturate the number of EMS induced mutations that can be tolerated by viable and fertile plants.
resultsBy mutagenizing with different EMS concentrations we have developed a permanent collection of 3712 M2/M3 independent mutant lines in the reference strain Landsberg erecta (Ler) of A. thaliana. This population has been named as the Arabidopsis TILLer collection. The frequency of mutations per line was maximized by using M1 plants with low but sufficient seed fertility. Application of TILLING to search for mutants in 14 genes identified 21 to 46 mutations per gene, which correspond to a total of 450 mutations. Missense mutations were found for all genes while truncations were selected for all except one. We estimated that, on average, these lines carry one mutation every 89 kb, Ler population providing a total of more than five million induced mutations. It is estimated that TILLer collection shows a two to three fold higher EMS mutation density per individual than previously reported A. thaliana population.
conclusionsAnalysis of TILLer collection demonstrates its usefulness for large scale TILLING reverse genetics in another reference genetic background of A. thaliana. Comparisons with TILLING populations in other organisms indicate that this new A. thaliana collection carries the highest chemically induced mutation density per individual known in diploid species.
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