ArticleMalaria journal2009
A comparative study of a flow-cytometry-based assessment of in vitro Plasmodium falciparum drug sensitivity.
Article in Malaria journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
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- Generation of a new DiCre expressing parasite strain for functional characterization of Plasmodium falciparum genes in blood stages.Scientific reports · 2024Article
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- Enhancing neutralization of Plasmodium falciparum using a novel monoclonal antibody against the rhoptry-associated membrane antigen.Scientific reports · 2022Article
- Compounds targeting GPI biosynthesis orComputational and structural biotechnology journal · 2022Article
- Progress and challenges in the use of fluorescence-based flow cytometric assays for anti-malarial drug susceptibility tests.Malaria journal · 2021Review
- Screening Marine Natural Products for New Drug Leads against Trypanosomatids and Malaria.Marine drugs · 2020Review
- Evaluating antimalarial efficacy by tracking glycolysis in Plasmodium falciparum using NMR spectroscopy.Scientific reports · 2018Article
- Quantification of Plasmodium ex vivo drug susceptibility by flow cytometry.Malaria journal · 2015Article
- Optimization of a multi-well colorimetric assay to determine haem species in Plasmodium falciparum in the presence of anti-malarials.Malaria journal · 2015Article
- Temporal changes in Plasmodium falciparum anti-malarial drug sensitivity in vitro and resistance-associated genetic mutations in isolates from Papua New Guinea.Malaria journal · 2015Article
- Drug repositioning as a route to anti-malarial drug discovery: preliminary investigation of the in vitro anti-malarial efficacy of emetine dihydrochloride hydrate.Malaria journal · 2013Article
- Amazonian plant natural products: perspectives for discovery of new antimalarial drug leads.Molecules (Basel, Switzerland) · 2013Review
- Defining the timing of action of antimalarial drugs against Plasmodium falciparum.Antimicrobial agents and chemotherapy · 2013Article
- Functionalized magnetic nanoparticles for the detection and quantitative analysis of cell surface antigen.BioMed research international · 2013Article
- Article
- A novel flow cytometric hemozoin detection assay for real-time sensitivity testing of Plasmodium falciparum.PloS one · 2013Article
- A whole cell pathway screen reveals seven novel chemosensitizers to combat chloroquine resistant malaria.Scientific reports · 2013Article
- A simple and inexpensive haemozoin-based colorimetric method to evaluate anti-malarial drug activity.Malaria journal · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRecently developed Sybr Green-based in vitro Plasmodium falciparum drug sensitivity assays provide an attractive alternative to current manual and automated methods. The present study evaluated flow cytometry measurement of DNA staining with Sybr Green in comparison with the P. falciparum lactate dehydrogenase assay, the tritiated hypoxanthine incorporation assay, a previously described Sybr Green based plate reader assay and light microscopy.
methodsAll assays were set up in standardized format in 96-well plates. The 50% inhibitory concentrations (IC50) of chloroquine, mefloquine and dihydroartemisinin against the laboratory adapted P. falciparum strains 3D7, E8B, W2mef and Dd2 were determined using each method.
resultsThe resolution achieved by flow cytometry allowed quantification of the increase in individual cell DNA content after an incubation period of only 24 h. Regression, and Bland and Altman analyses showed that the IC50 values determined using the flow cytometry assay after 24 h agreed well with those obtained using the hypoxanthine incorporation assay, the P. falciparum lactate dehydrogenase assay, the Sybr Green plate reader assay and light microscopy. However the values obtained with the flow cytometry assay after 48 h of incubation differed significantly from those obtained with the hypoxanthine incorporation assay, and the P. falciparum lactate dehydrogenase assay at low IC50 values, but agreed well with the Sybr Green plate reader assay and light microscopy.
conclusionsAlthough flow cytometric equipment is expensive, the necessary reagents are inexpensive, the procedure is simple and rapid, and the cell volume required is minimal. This should allow field studies using fingerprick sample volumes.
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