ArticleBMC microbiology2009
The low-affinity phosphate transporter PitA is dispensable for in vitro growth of Mycobacterium smegmatis.
Article in BMC microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
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- Marine picocyanobacterial PhnD1 shows specificity for various phosphorus sources but likely represents a constitutive inorganic phosphate transporter.The ISME journal · 2023Article
- Phosphite synthetic auxotrophy as an effective biocontainment strategy for the industrial chassis Pseudomonas putida.Microbial cell factories · 2022Article
- Analysis of the Genome of the Heavy Metal Resistant and Hydrocarbon-Degrading RhizosphericInternational journal of molecular sciences · 2021Article
- Genomic Analysis ofG3 (Bethesda, Md.) · 2019Article
- Duplication of genes in an ATP-binding cassette transport system increases dynamic range while maintaining ligand specificity.The Journal of biological chemistry · 2014Article
- The complete genome sequence of the plant growth-promoting bacterium Pseudomonas sp. UW4.PloS one · 2013Article
- Ecophysiology of an ammonia-oxidizing archaeon adapted to low-salinity habitats.Microbial ecology · 2012Article
- Deciphering the genome of polyphosphate accumulating actinobacterium Microlunatus phosphovorus.DNA research : an international journal for rapid publication of reports on genes and genomes · 2012Article
- Gene expression profiling of Mycobacterium avium subsp. paratuberculosis in simulated multi-stress conditions and within THP-1 cells reveals a new kind of interactive intramacrophage behaviour.BMC microbiology · 2012Article
- A VapBC toxin-antitoxin module is a posttranscriptional regulator of metabolic flux in mycobacteria.Journal of bacteriology · 2012Article
- Uptake of sulfate but not phosphate by Mycobacterium tuberculosis is slower than that for Mycobacterium smegmatis.Journal of bacteriology · 2012Article
- Thalassospira sp. isolated from the oligotrophic eastern Mediterranean Sea exhibits chemotaxis toward inorganic phosphate during starvation.Applied and environmental microbiology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMycobacteria have been shown to contain an apparent redundancy of high-affinity phosphate uptake systems, with two to four copies of such systems encoded in all mycobacterial genomes sequenced to date. In addition, all mycobacteria also contain at least one gene encoding the low-affinity phosphate transporter, Pit. No information is available on a Pit system from a Gram-positive microorganism, and the importance of this system in a background of multiple other phosphate transporters is unclear.
resultsThe aim of this study was to determine the physiological role of the PitA phosphate transporter in Mycobacterium smegmatis. Expression of pitA was found to be constitutive under a variety of growth conditions. An unmarked deletion mutant in pitA of M. smegmatis was created. The deletion did not affect in vitro growth or phosphate uptake of M. smegmatis. Expression of the high-affinity transporters, PstSCAB and PhnDCE, was increased in the pitA deletion strain.
conclusionPitA is the only low-affinity phosphate transport system annotated in the genome of M. smegmatis. The lack of phenotype of the pitA deletion strain shows that this system is dispensable for in vitro growth of this organism. However, increased expression of the remaining phosphate transporters in the mutant indicates a compensatory mechanism and implies that PitA is indeed used for the uptake of phosphate in M. smegmatis.
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