ArticleBMC cell biology2009
Disruption of occludin function in polarized epithelial cells activates the extrinsic pathway of apoptosis leading to cell extrusion without loss of transepithelial resistance.
Article in BMC cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Enhancement of intestinal barrier function and alleviation of mycophenolic acid toxicity by a probiotic-conditioned mediumFrontiers in nutrition · 2026Article
- Nor1 and Mitophagy: An Insight into Sertoli Cell Function Regulating Spermatogenesis Using a Transgenic Rat Model.International journal of molecular sciences · 2025Article
- Intestinal Epithelial Tight Junction Barrier Regulation by Novel Pathways.Inflammatory bowel diseases · 2025Review
- RhoGDI1 regulates cell-cell junctions in polarized epithelial cells.Frontiers in cell and developmental biology · 2024Article
- Tight junction proteins occludin and ZO-1 as regulators of epithelial proliferation and survival.Annals of the New York Academy of Sciences · 2022Review
- Concerted action of berberine in the porcine intestinal epithelial model IPEC-J2: Effects on tight junctions and apoptosis.Physiological reports · 2022Article
- An Overview of Physical, Microbiological and Immune Barriers of Oral Mucosa.International journal of molecular sciences · 2021Review
- TRAIL receptor-induced features of epithelial-to-mesenchymal transition increase tumour phenotypic heterogeneity: potential cell survival mechanisms.British journal of cancer · 2021Review
- Occludin protects secretory cells from ER stress by facilitating SNARE-dependent apical protein exocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- LRRC75A antisense lncRNA1 knockout attenuates inflammatory responses of bovine mammary epithelial cells.International journal of biological sciences · 2020Article
- Inflammation-induced Occludin Downregulation Limits Epithelial Apoptosis by Suppressing Caspase-3 Expression.Gastroenterology · 2019Article
- Article
- Homeostatic Signaling by Cell-Cell Junctions and Its Dysregulation during Cancer Progression.Journal of clinical medicine · 2016Review
- Epithelial adhesion molecules and the regulation of intestinal homeostasis during neutrophil transepithelial migration.Tissue barriers · 2015Article
- The role of tight junctions in mammary gland function.Journal of mammary gland biology and neoplasia · 2014Review
- Collective migration of cancer-associated fibroblasts is enhanced by overexpression of tight junction-associated proteins claudin-11 and occludin.Molecular oncology · 2014Article
- Intestinal epithelial apoptosis initiates gut mucosal injury during extracorporeal membrane oxygenation in the newborn piglet.Laboratory investigation; a journal of technical methods and pathology · 2014Article
- Article
- Occludin is required for apoptosis when claudin-claudin interactions are disrupted.Cell death & disease · 2012Article
- Apoptotic regulation of epithelial cellular extrusion.Apoptosis : an international journal on programmed cell death · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundOccludin is a tetraspanin protein normally localized to tight junctions. The protein interacts with a variety of pathogens including viruses and bacteria, an interaction that sometimes leads to its extrajunctional localization.
resultsHere we report that treatment of mammary epithelial monolayers with a circularized peptide containing a four amino acid sequence found in the second extracellular loop of occludin, LHYH, leads to the appearance of extrajunctional occludin and activation of the extrinsic apoptotic pathway. At early times after peptide treatment endogenous occludin and the LYHY peptide were co-localized in extrajunctional patches, which were also shown to contain components of the death inducing signaling complex (DISC), caspases 8 and 3, the death receptor FAS and the adaptor molecule FADD. After this treatment occludin could be immunoprecipitated with FADD, confirming its interaction with the DISC. Extrusion after LYHY treatment was accomplished with no loss of epithelial resistance.
conclusionThese observations provide strong evidence that, following disruption, occludin forms a complex with the extrinsic death receptor leading to extrusion of apoptotic cells from the epithelial monolayer. They suggest that occludin has a protective as well as a barrier forming role in epithelia; pathogenic agents which utilize this protein as an entry point into the cell might set off an apoptotic reaction allowing extrusion of the infected cell before the pathogen can gain entry to the interstitial space.
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Registered trials
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