ArticleJournal of thrombosis and haemostasis : JTH2010
Mlck1a is expressed in zebrafish thrombocytes and is an essential component of thrombus formation.
Article in Journal of thrombosis and haemostasis : JTH, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Zebrafish as a Model for Cardiovascular and Metabolic Disease: The Future of Precision Medicine.Biomedicines · 2024Review
- Single-cell transcriptome reveals insights into the development and function of the zebrafish ovary.eLife · 2022Article
- Zebrafish for thrombocytopoiesis- and hemostasis-related researches and disorders.Blood science (Baltimore, Md.) · 2020Review
- Epicardium in Heart Development.Cold Spring Harbor perspectives in biology · 2020Review
- Generation of transgenic zebrafish with 2 populations of RFP- and GFP-labeled thrombocytes: analysis of their lipids.Blood advances · 2019Article
- The epicardium as a hub for heart regeneration.Nature reviews. Cardiology · 2018Review
- Functional Genomics for the Identification of Modulators of Platelet-Dependent Thrombus Formation.TH open : companion journal to thrombosis and haemostasis · 2018Review
- Zebrafish thrombocytes: functions and origins.Advances in hematology · 2012Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundWe have used the advantages of the zebrafish model system to demonstrate which of the vertebrate myosin light chain kinase (MLCK) genes is expressed in thrombocytes and important for thrombus formation. METHODS AND
resultsHere we report that Mlck1a is an essential component of thrombus formation. Phylogenetic data revealed four zebrafish orthologous for three human MLCK genes. To investigate expression of the zebrafish mlck genes in thrombocytes we compared GFP-tagged platelets with other cells by microarray analysis, and showed that mlck1a expression was 4.5-fold enriched in platelets. Furthermore, mlck1a mRNA and mRNA for the platelet-specific cd41 co-localized in thrombi. Expression of other mlck subtypes was lower in GFP-tagged platelets (mlck1b; 0.77-fold enriched) and absent in thrombi (mlck1b, -2, -3). To investigate the role of Mlck1a in thrombus formation, we knocked down mlck1a using two morpholinos. This resulted in impaired morphology changes of platelets adhering on fibrinogen. In a thrombosis model, in which thrombocytes adhere to the vessel wall damaged by laser irradiation, thrombus formation was slowed down in mlck1a-deficient embryos.
conclusionWe conclude that Mlck1a is the subtype of MLCK that contributes to platelet shape change and thrombus formation.
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