ArticleThe Biochemical journal2010
Post-transcriptional regulation of MEK-1 by polyamines through the RNA-binding protein HuR modulating intestinal epithelial apoptosis.
Article in The Biochemical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 61 citations in OpenAlex.
- Sm-site containing mRNAs can accept Sm-rings and are downregulated in Spinal Muscular Atrophy.Nucleic acids research · 2025Article
- Research progress on the microbial metabolism and transport of polyamines and their roles in animal gut homeostasis.Journal of animal science and biotechnology · 2025Review
- Nurturing gut health: role of m6A RNA methylation in upholding the intestinal barrier.Cell death discovery · 2024Review
- Stabilization ofAmerican journal of physiology. Gastrointestinal and liver physiology · 2023Article
- miR-542-5p targets c-myc and negates the cell proliferation effect of SphK1 in intestinal epithelial cells.American journal of physiology. Cell physiology · 2023Article
- Extracellular riboflavin induces anaerobic biofilm formation in Shewanella oneidensis.Biotechnology for biofuels · 2021Article
- TRPC1-mediated CaPhysiological reports · 2021Article
- RNA-seq of human T cells after hematopoietic stem cell transplantation identifiesScience translational medicine · 2021Article
- Molecular Targeting of HuR Oncoprotein Suppresses MITF and Induces Apoptosis in Melanoma Cells.Cancers · 2021Article
- Polyamines and related signaling pathways in cancer.Cancer cell international · 2020Review
- Polyamines in Gut Epithelial Renewal and Barrier Function.Physiology (Bethesda, Md.) · 2020Review
- Growth Inhibitory Signaling of the Raf/MEK/ERK Pathway.International journal of molecular sciences · 2020Review
- RNA-binding protein HuR regulates translation of vitamin D receptor modulating rapid epithelial restitution after wounding.American journal of physiology. Cell physiology · 2020Article
- Glycyrrhetinic Acid Maintains Intestinal Homeostasis via HuR.Frontiers in pharmacology · 2019Article
- Polyamine Deacetylase Structure and Catalysis: Prokaryotic Acetylpolyamine Amidohydrolase and Eukaryotic HDAC10.Biochemistry · 2018Review
- Putrescine as indicator of manganese neurotoxicity: Dose-response study in human SH-SY5Y cells.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2018Article
- Cooperative Repression of Insulin-Like Growth Factor Type 2 Receptor Translation by MicroRNA 195 and RNA-Binding Protein CUGBP1.Molecular and cellular biology · 2017Article
- HuR Enhances Early Restitution of the Intestinal Epithelium by Increasing Cdc42 Translation.Molecular and cellular biology · 2017Article
- Posttranscriptional regulation of 14-3-3ζ by RNA-binding protein HuR modulating intestinal epithelial restitution after wounding.Physiological reports · 2016Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
MEK-1 [MAPK (mitogen-activated protein kinase) kinase-1] is an important signal transducing enzyme that is implicated in many aspects of cellular functions. In the present paper, we report that cellular polyamines regulate MEK-1 expression at the post-transcriptional level through the RNA-binding protein HuR (Hu-antigen R) in IECs (intestinal epithelial cells). Decreasing the levels of cellular polyamines by inhibiting ODC (ornithine decarboxylase) stabilized MEK-1 mRNA and promoted its translation through enhancement of the interaction between HuR and the 3'-untranslated region of MEK-1 mRNA, whereas increasing polyamine levels by ectopic ODC overexpression destabilized the MEK-1 transcript and repressed its translation by reducing the abundance of HuR-MEK-1 mRNA complex; neither intervention changed MEK-1 gene transcription via its promoter. HuR silencing rendered the MEK-1 mRNA unstable and inhibited its translation, thus preventing increases in MEK-1 mRNA and protein in polyamine-deficient cells. Conversely, HuR overexpression increased MEK-1 mRNA stability and promoted its translation. Inhibition of MEK-1 expression by MEK-1 silencing or HuR silencing prevented the increased resistance of polyamine-deficient cells to apoptosis. Moreover, HuR overexpression did not protect against apoptosis if MEK-1 expression was silenced. These results indicate that polyamines destabilize the MEK-1 mRNA and repress its translation by inhibiting the association between HuR and the MEK-1 transcript. Our findings indicate that MEK-1 is a key effector of the HuR-elicited anti-apoptotic programme in IECs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.