ArticleTissue engineering. Part C, Methods2010
Bioengineered three-dimensional physiological model of colonic longitudinal smooth muscle in vitro.
Article in Tissue engineering. Part C, Methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- An exploratory in vitro co-culture of enteric neurons and smooth muscle cells demonstrates neuronal contribution to muscle layer formation.Scientific reports · 2026Article
- Persistent enteric neuroinflammation chronically impairs colonic motility in a pyridostigmine bromide-induced mouse model of Gulf War illness.Biology open · 2025Article
- Crisis in the gut: navigating gastrointestinal challenges in Gulf War Illness with bioengineering.Military Medical Research · 2024Review
- In Vitro Tumor Models on Chip and Integrated Microphysiological Analysis Platform (MAP) for Life Sciences and High-Throughput Drug Screening.Biosensors · 2023Review
- Tissue Engineering for Gastrointestinal and Genitourinary Tracts.International journal of molecular sciences · 2022Review
- BioSphincters to treat Fecal Incontinence in Nonhuman Primates.Scientific reports · 2019Article
- Bioengineering and regeneration of gastrointestinal tissue: where are we now and what comes next?Expert opinion on biological therapy · 2019Review
- Bioengineering the gut: future prospects of regenerative medicine.Nature reviews. Gastroenterology & hepatology · 2016Review
- The influence of extracellular matrix composition on the differentiation of neuronal subtypes in tissue engineered innervated intestinal smooth muscle sheets.Biomaterials · 2014Article
- Tissue engineering in the gut: developments in neuromusculature.Gastroenterology · 2014Review
- Bioengineering of physiologically functional intrinsically innervated human internal anal sphincter constructs.Tissue engineering. Part A · 2014Article
- Design strategies of biodegradable scaffolds for tissue regeneration.Biomedical engineering and computational biology · 2014Review
- Tissue engineering and regenerative medicine as applied to the gastrointestinal tract.Current opinion in biotechnology · 2013Review
- Regenerative surgery: tissue engineering in general surgical practice.World journal of surgery · 2012Review
- Chitosan-based scaffolds for the support of smooth muscle constructs in intestinal tissue engineering.Biomaterials · 2012Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe objective of this study was to develop a physiological model of longitudinal smooth muscle tissue from isolated longitudinal smooth muscle cells arranged in the longitudinal axis.
methodsLongitudinal smooth muscle cells from rabbit sigmoid colon were isolated and expanded in culture. Cells were seeded at high densities onto laminin-coated Sylgard surfaces with defined wavy microtopographies. A highly aligned cell sheet was formed, to which addition of fibrin resulted in delamination.
results(1) Acetylcholine (ACh) induced a dose-dependent, rapid, and sustained force generation. (2) Absence of extracellular calcium attenuated the magnitude and sustainability of ACh-induced force by 50% and 60%, respectively. (3) Vasoactive intestinal peptide also attenuated the magnitude and sustainability of ACh-induced force by 40% and 60%, respectively. These data were similar to force generated by longitudinal tissue. (4) Bioengineered constructs also maintained smooth muscle phenotype and calcium-dependence characteristics. SUMMARY: This is a novel physiologically relevant in vitro three-dimensional model of colonic longitudinal smooth muscle tissue. Bioengineered three-dimensional longitudinal smooth muscle presents the ability to generate force, and respond to contractile agonists and relaxant peptides similar to native longitudinal tissue. This model has potential applications to investigate the underlying pathophysiology of dysfunctional colonic motility. It also presents as a readily implantable band-aid colonic longitudinal muscle tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.