ArticleTissue engineering. Part A2010
Three-dimensional culture alters primary cardiac cell phenotype.
Article in Tissue engineering. Part A, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 47 citations in OpenAlex.
- High density sphere culture of adult cardiac cells increases the levels of cardiac and progenitor markers and shows signs of vasculogenesis.BioMed research international · 2013Trial
- Evaluating Seeding Density Effects on Cardiac Organoid Health and Functionality for Toxicity Studies.Tissue engineering. Part A · 2026Article
- Mechanical maturation of human dermal fibroblast-laden microporous annealed particle scaffolds during long-term in vitro culture.Acta biomaterialia · 2025Article
- Beyond pharmaceuticals: Fit-for-purpose new approach methodologies for environmental cardiotoxicity testing.ALTEX · 2023Review
- Enhancing Peptide Biomaterials for Biofabrication.Polymers · 2021Review
- Superiority of focused ion beam-scanning electron microscope tomography of cardiomyocytes over standard 2D analyses highlighted by unmasking mitochondrial heterogeneity.Journal of cachexia, sarcopenia and muscle · 2021Article
- In vitro and in vivo analysis of visible light crosslinkable gelatin methacryloyl (GelMA) hydrogels.Biomaterials science · 2017Article
- Tissue specific microenvironments: a key tool for tissue engineering and regenerative medicine.Journal of biological engineering · 2017Review
- IGF1 and NRG1 Enhance Proliferation, Metabolic Maturity, and the Force-Frequency Response in hESC-Derived Engineered Cardiac Tissues.Stem cells international · 2017Article
- Advances in cell culture: anchorage dependence.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Multi-cellular interactions sustain long-term contractility of human pluripotent stem cell-derived cardiomyocytes.American journal of translational research · 2014Article
- 3D engineered cardiac tissue models of human heart disease: learning more from our mice.Trends in cardiovascular medicine · 2013Review
- 3D-culture system for heart regeneration and cardiac medicine.BioMed research international · 2013Review
- The expanding world of tissue engineering: the building blocks and new applications of tissue engineered constructs.IEEE reviews in biomedical engineering · 2013Review
- 26S proteasome regulation of Ankrd1/CARP in adult rat ventricular myocytes and human microvascular endothelial cells.Biochemical and biophysical research communications · 2012Article
- Article
- The multiparametric effects of hydrodynamic environments on stem cell culture.Tissue engineering. Part B, Reviews · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The directed formation of complex three-dimensional (3D) tissue architecture is a fundamental goal in tissue engineering and regenerative medicine. The growth of cells in 3D structures is expected to influence cellular phenotype and function, especially relative cell distribution, expression profiles, and responsiveness to exogenous signals; however, relatively few studies have been carried out to examine the effects of 3D reaggregation on cells from critical target organs, like the heart. Accordingly, we cultured primary cardiac ventricular cells in a 3D model system using a serum-free medium to test the hypothesis that expression profiles, multicellular organizational pathways, tissue maturation markers, and responsiveness to hormone stimulation were significantly altered in stable cell populations grown in 3D versus 2D culture. We found that distinct multi-cellular structures formed in 3D in conjunction with changes in mRNA expression profile, up-regulation of endothelial cell migratory pathways, decreases in the expression of fetal genes (Nppa and Ankrd1), and increased sensitivity to tri-iodothyronine stimulation when compared to parallel 2D cultures comprising the same cell populations. These results indicate that the culture of primary cardiac cells in 3D aggregates leads to physiologically relevant alterations in component cell phenotype consistent with cardiac ventricular tissue formation and maturation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.