ArticleAnalytical chemistry2010
On-chip activation and subsequent detection of individual antigen-specific T cells.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Measuring single-cell protein secretion in immunology: Technologies, advances, and applications.European journal of immunology · 2021Review
- Biomaterials as Tools to Decode Immunity.Advanced materials (Deerfield Beach, Fla.) · 2020Review
- Protein diffusion from microwells with contrasting hydrogel domains.APL bioengineering · 2019Article
- Current Trends of Microfluidic Single-Cell Technologies.International journal of molecular sciences · 2018Review
- Frequency and reactivity of antigen-specific T cells were concurrently measured through the combination of artificial antigen-presenting cell, MACS and ELISPOT.Scientific reports · 2017Article
- Immune cell profiling in cancer: molecular approaches to cell-specific identification.NPJ precision oncology · 2017Review
- Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization.Sensors (Basel, Switzerland) · 2016Review
- Protein adsorption in microengraving immunoassays.Sensors (Basel, Switzerland) · 2015Article
- Analysis of single-cell cytokine secretion reveals a role for paracrine signaling in coordinating macrophage responses to TLR4 stimulation.Science signaling · 2015Article
- Characterization of cell seeding and specific capture of B cells in microbubble well arrays.Biomedical microdevices · 2013Article
- New assays for monitoring residual HIV burden in effectively treated individuals.Current opinion in HIV and AIDS · 2013Review
- Functional single-cell analysis of T-cell activation by supported lipid bilayer-tethered ligands on arrays of nanowells.Lab on a chip · 2013Article
- Autoimmune T-cell reactivity to myelin proteolipids and glycolipids in multiple sclerosis.Multiple sclerosis international · 2013Review
- Cellular barcodes for efficiently profiling single-cell secretory responses by microengraving.Analytical chemistry · 2012Article
- MHC class II tetramers.Journal of immunology (Baltimore, Md. : 1950) · 2012Review
- Rapid, efficient functional characterization and recovery of HIV-specific human CD8+ T cells using microengraving.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Imaging immune surveillance of individual natural killer cells confined in microwell arrays.PloS one · 2010Article
- Peptide-MHC cellular microarray with innovative data analysis system for simultaneously detecting multiple CD4 T-cell responses.PloS one · 2010Article
- Multidimensional analysis of the frequencies and rates of cytokine secretion from single cells by quantitative microengraving.Lab on a chip · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
The frequencies of antigen-specific CD4+ T cells in samples of human tissue have been difficult to determine accurately ex vivo, particularly for autoimmune diseases such as multiple sclerosis or type 1 diabetes. Conventional approaches involve the expansion of primary T cells in vitro to increase the numbers of cells, and a subsequent assessment of the frequencies of antigen-specific T cells in the expanded population by limiting dilution or by using fluorescently labeled tetramers of peptide-loaded major histocompatibility complex (MHC) receptors. Here we describe an alternative approach that uses arrays of subnanoliter wells coated with recombinant peptide-loaded MHC class II monomers to isolate and stimulate individual CD4+ T cells in an antigen-specific manner. In these experiments, activation was monitored using microengraving to capture two cytokines (IFNgamma and IL-17) released from single cells. This new method should enable direct enumeration of antigen-specific CD4+ T cells ex vivo from clinical samples.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.