ArticleJournal of the American Chemical Society2010
Multiparameter screening on SlipChip used for nanoliter protein crystallization combining free interface diffusion and microbatch methods.
Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 93 citations in OpenAlex.
- Technical Scale in Biomolecular Crystallization.Advances in biochemical engineering/biotechnology · 2026Review
- A Robust Normally Closed Pneumatic Valve for Integrated Microfluidic Flow Control.Micromachines · 2024Article
- Advancing microfluidic diagnostic chips into clinical use: a review of current challenges and opportunities.Lab on a chip · 2022Review
- Nanomaterial-assisted microfluidics for multiplex assays.Mikrochimica acta · 2022Review
- Slip-driven microfluidic devices for nucleic acid analysis.Biomicrofluidics · 2019Review
- Correlative imaging across microscopy platforms using the fast and accurate relocation of microscopic experimental regions (FARMER) method.The Review of scientific instruments · 2017Article
- Using flow technologies to direct the synthesis and assembly of materials in solution.Chemistry Central journal · 2017Review
- Capture and X-ray diffraction studies of protein microcrystals in a microfluidic trap array.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Individually addressable arrays of replica microbial cultures enabled by splitting SlipChips.Integrative biology : quantitative biosciences from nano to macro · 2014Article
- Nanoliter-scale protein crystallization and screening with a microfluidic droplet robot.Scientific reports · 2014Article
- An overview of biological macromolecule crystallization.International journal of molecular sciences · 2013Review
- The potential impact of droplet microfluidics in biology.Analytical chemistry · 2013Article
- Control of initiation, rate, and routing of spontaneous capillary-driven flow of liquid droplets through microfluidic channels on SlipChip.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- The Protein Maker: an automated system for high-throughput parallel purification.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- Structural genomics of infectious disease drug targets: the SSGCID.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- Digital isothermal quantification of nucleic acids via simultaneous chemical initiation of recombinase polymerase amplification reactions on SlipChip.Analytical chemistry · 2011Article
- Microfluidic technologies for synthetic biology.International journal of molecular sciences · 2011Review
- Digital PCR on a SlipChip.Lab on a chip · 2010Article
- Nanovolume optimization of protein crystal growth using the microcapillary protein crystallization system.Journal of applied crystallography · 2010Article
- Dead-end filling of SlipChip evaluated theoretically and experimentally as a function of the surface chemistry and the gap size between the plates for lubricated and dry SlipChips.Langmuir : the ACS journal of surfaces and colloids · 2010Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
This paper describes two SlipChip-based approaches to protein crystallization: a SlipChip-based free interface diffusion (FID) method and a SlipChip-based composite method that simultaneously performs microbatch and FID crystallization methods in a single device. The FID SlipChip was designed to screen multiple reagents, each at multiple diffusion equilibration times, and was validated by screening conditions for crystallization of two proteins, enoyl-CoA hydratase from Mycobacterium tuberculosis and dihydrofolate reductase/thymidylate synthase from Babesia bovis, against 48 different reagents at five different equilibration times each, consuming 12 microL of each protein for a total of 480 experiments using three SlipChips. The composite SlipChip was designed to screen multiple reagents, each at multiple mixing ratios and multiple equilibration times, and was validated by screening conditions for crystallization of two proteins, enoyl-CoA hydratase from Mycobacterium tuberculosis and dihydrofolate reductase/thymidylate synthase from Babesia bovis. To prevent cross-contamination while keeping the solution in the neck channels for FID stable, the plates of the SlipChip were etched with a pattern of nanowells. This nanopattern was used to increase the contact angle of aqueous solutions on the surface of the silanized glass. The composite SlipChip increased the number of successful crystallization conditions and identified more conditions for crystallization than separate FID and microbatch screenings. Crystallization experiments were scaled up in well plates using conditions identified during the SlipChip screenings, and X-ray diffraction data were obtained to yield the protein structure of dihydrofolate reductase/thymidylate synthase at 1.95 A resolution. This free-interface diffusion approach provides a convenient and high-throughput method of setting up gradients in microfluidic devices and may find additional applications in cell-based assays.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.