Evidence map›Paper›PMID 20000708›Full record

ArticleJournal of the American Chemical Society2010

User-loaded SlipChip for equipment-free multiplexed nanoliter-scale experiments.

Liang Li, Wenbin Du, Rustem Ismagilov

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
4.8field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 74 citations in OpenAlex.

  1. Article
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  13. Individually addressable arrays of replica microbial cultures enabled by splitting SlipChips.Integrative biology : quantitative biosciences from nano to macro · 2014
    Article
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  19. Article
  20. Structural genomics of infectious disease drug targets: the SSGCID.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Liang LiDepartment of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
Wenbin Du
Rustem Ismagilov
University of Chicago · US

Funding

Sub-project 5U54GM074961 · NIGMS · EMERALD BIOSTRUCTURES · PI KUHN, PETER · 2005 to 2009
$19.3M
2007 NIH Director's Pioneer Award Program (DP1)DP1OD003584 · OD · UNIVERSITY OF CHICAGO · PI ISMAGILOV, RUSTEM F · 2007 to 2011
$3.9M
Enabling Nanoliter Membrane Protein Crystallization(RMI)R01GM075827 · NIGMS · UNIVERSITY OF CHICAGO · PI ISMAGILOV, RUSTEM F · 2005 to 2009
$1.1M
NCI NIH HHS Y1-CO-1020NIAID NIH HHS HHSN272200700057CNIGMS NIH HHS R01 GM075827NIGMS NIH HHS U54 GM074961NIGMS NIH HHS Y1-GM-1104NIH HHS 1DP1OD003584NIH HHS DP1 OD003584PHS HHS HHSN-272200700057C
6 · The paper itself

Abstract

This paper describes a microfluidic approach to perform multiplexed nanoliter-scale experiments by combining a sample with multiple different reagents, each at multiple mixing ratios. This approach employs a user-loaded, equipment-free SlipChip. The mixing ratios, characterized by diluting a fluorescent dye, could be controlled by the volume of each of the combined wells. The SlipChip design was validated on an approximately 12 nL scale by screening the conditions for crystallization of glutaryl-CoA dehydrogenase from Burkholderia pseudomallei against 48 different reagents; each reagent was tested at 11 different mixing ratios, for a total of 528 crystallization trials. The total consumption of the protein sample was approximately 10 microL. Conditions for crystallization were successfully identified. The crystallization experiments were successfully scaled up in well plates using the conditions identified in the SlipChip. Crystals were characterized by X-ray diffraction and provided a protein structure in a different space group and at a higher resolution than the structure obtained by conventional methods. In this work, this user-loaded SlipChip has been shown to reliably handle fluids of diverse physicochemical properties, such as viscosities and surface tensions. Quantitative measurements of fluorescent intensities and high-resolution imaging were straighforward to perform in these glass SlipChips. Surface chemistry was controlled using fluorinated lubricating fluid, analogous to the fluorinated carrier fluid used in plug-based crystallization. Thus, we expect this approach to be valuable in a number of areas beyond protein crystallization, especially those areas where droplet-based microfluidic systems have demonstrated successes, including measurements of enzyme kinetics and blood coagulation, cell-based assays, and chemical reactions.

Indexed as

Burkholderia pseudomalleiCrystallizationGlutaryl-CoA DehydrogenaseHyphomicrobiaceaeMicrofluidic Analytical TechniquesNanotechnologyPhotosynthetic Reaction Center Complex ProteinsReproducibility of ResultsGlutaryl-CoA DehydrogenasePhotosynthetic Reaction Center Complex Proteins

Identifiers

PMID20000708
PMCPMC2802657
OpenAlexW1970297152

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.