ArticleAnalytical chemistry2010
Accurate MALDI-TOF/TOF sequencing of one-bead-one-compound peptide libraries with application to the identification of multiligand protein affinity agents using in situ click chemistry screening.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation.Analytical chemistry · 2022Article
- Peptide-Peptide Co-Assembly: A Design Strategy for Functional Detection of C-peptide, A Biomarker of Diabetic Neuropathy.International journal of molecular sciences · 2020Article
- Protein-Catalyzed Capture Agents.Chemical reviews · 2019Review
- Library Design-Facilitated High-Throughput Sequencing of Synthetic Peptide Libraries.ACS combinatorial science · 2017Article
- High-Throughput Approaches to the Development of Molecular Imaging Agents.Molecular imaging and biology · 2017Review
- Screening and identification of mimotopes of the major shrimp allergen tropomyosin using one-bead-one-compound peptide libraries.Cellular & molecular immunology · 2017Article
- An efficient strategy to enhance binding affinity and specificity of a known isozyme inhibitor.Organic & biomolecular chemistry · 2016Article
- A protein-targeting strategy used to develop a selective inhibitor of the E17K point mutation in the PH domain of Akt1.Nature chemistry · 2015Article
- Investigating fluorescent dyes in fluorescence-assisted screenings.Chemical communications (Cambridge, England) · 2014Article
- Observation of the controlled assembly of preclick components in the in situ click chemistry generation of a chitinase inhibitor.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Click chemistry facilitates formation of reporter ions and simplified synthesis of amine-reactive multiplexed isobaric tags for protein quantification.Journal of the American Chemical Society · 2012Article
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Authors and funding
7 authors.
Funding
Abstract
Combinatorial one-bead-one-compound (OBOC) peptide libraries are widely used for affinity screening, and the sequencing of peptides from hit beads is a key step in the process. For rapid sequencing, CNBr cleavage of the peptides from the beads, followed by de novo sequencing by MALDI-TOF/TOF, is explored. We report on a semiautomated sequencing algorithm and validate it through comparison against Edman degradation sequencing. The initial 44% sequencing success rate of the standard de novo sequencing software was improved to nearly 100%. The sequencing algorithm incorporates existing knowledge of amino acid chemistry and a new strategy for differentiating isobaric amino acids. We tested the algorithm by using MALDI-TOF/TOF to identify a peptide biligand affinity agent against the protein bovine carbonic anhydrase II, starting from comprehensive one-bead-one-compound peptide libraries comprised of non-natural and artificial amino acid components and using the strategy of in situ click/OBOC library screening.
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Registered trials
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