ArticleMethods in molecular biology (Clifton, N.J.)2010
In vitro footprinting of promoter regions within supercoiled plasmid DNA.
Article in Methods in molecular biology (Clifton, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Impact of G-Quadruplexes on the Regulation of Genome Integrity, DNA Damage and Repair.Biomolecules · 2021Review
- Article
- G-quadruplex deconvolution with physiological mimicry enhances primary screening: Optimizing the FRET MeltBiochimica et biophysica acta. Gene regulatory mechanisms · 2020Article
- Effects of 5-Hydroxymethylcytosine Epigenetic Modification on the Stability and Molecular Recognition of VEGF i-Motif and G-Quadruplex Structures.Journal of nucleic acids · 2018Article
- The effects of DNA supercoiling on G-quadruplex formation.Nucleic acids research · 2017Article
- Possible regulatory roles of promoter g-quadruplexes in cardiac function-related genes - human TnIc as a model.PloS one · 2013Article
- Yin Yang 1 contains G-quadruplex structures in its promoter and 5'-UTR and its expression is modulated by G4 resolvase 1.Nucleic acids research · 2012Article
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Abstract
Polypurine/polypyrimidine (pPu/pPy) tracts, which exist in the promoter regions of many growth-related genes, have been proposed to be very dynamic in their conformation. In this chapter, we describe a detailed protocol for DNase I and S1 nuclease footprinting experiments with supercoiled plasmid DNA containing the promoter regions to probe whether there are conformational transitions to B-type DNA, melted DNA, and G-quadruplex structures within this tract. This is demonstrated with the proximal promoter region of the human vascular endothelial growth factor (VEGF) gene, which also contains multiple binding sites for Sp1 and Egr-1 transcription factors.
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