ArticlePloS one2009
Temporal control of immediate early gene induction by light.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 86 citations in OpenAlex.
- Article
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- The dorsal column nuclei scale mechanical sensitivity in naive and neuropathic pain states.Cell reports · 2025Article
- Neuronal activity-dependent gene expression is stimulus-specific and changes with neuronal maturation.Frontiers in molecular neuroscience · 2025Article
- Combined-electrical optogenetic stimulation but not channelrhodopsin kinetics improves the fidelity of high rate stimulation in the auditory pathway in mice.Scientific reports · 2024Article
- Lactoferrin Modulates Induction of Transcription Factor c-Fos in Neuronal Cultures.International journal of molecular sciences · 2023Article
- Spike-timing-dependent plasticity rewards synchrony rather than causality.Cerebral cortex (New York, N.Y. : 1991) · 2022Article
- ΔFosB accumulation in hippocampal granule cells drives cFos pattern separation during spatial learning.Nature communications · 2022Article
- Article
- Memory trace interference impairs recall in a mouse model of Alzheimer's disease.Nature neuroscience · 2020Article
- TrpM8-mediated somatosensation in mouse neocortex.The Journal of comparative neurology · 2018Article
- Rhodopsin optogenetic toolbox v2.0 for light-sensitive excitation and inhibition in Caenorhabditis elegans.PloS one · 2018Article
- Article
- Breathing control center neurons that promote arousal in mice.Science (New York, N.Y.) · 2017Article
- Complex Photochemistry within the Green-Absorbing Channelrhodopsin ReaChR.Biophysical journal · 2017Article
- Hearing Loss Controlled by Optogenetic Stimulation of Nonexcitable Nonglial Cells in the Cochlea of the Inner Ear.Frontiers in molecular neuroscience · 2017Article
- Neuronal networks and mediators of cortical neurovascular coupling responses in normal and altered brain states.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Paradox of pattern separation and adult neurogenesis: A dual role for new neurons balancing memory resolution and robustness.Neurobiology of learning and memory · 2016Review
- Exploring Memory Representations with Activity-Based Genetics.Cold Spring Harbor perspectives in biology · 2015Review
- CBP-Dependent memory consolidation in the prefrontal cortex supports object-location learning.Hippocampus · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe light-gated cation channel channelrhodopsin-2 (ChR2) is a powerful tool for the optical induction of action potentials in neurons. Mutations of the cysteine 128 (C128) residue have been shown to greatly extend the lifetime of the conducting state of ChR2. However, until now, only subthreshold depolarizations have been reported from C128 mutants. METHODS AND
findingsHere we report the induction of long high-frequency spike trains by brief light pulses in ChR2(C128A)-transfected pyramidal cells in hippocampal slice culture. ChR2(C128A)-mediated spike bursts triggered expression of the immediate early gene c-fos in pyramidal neurons. Robust and cell-specific expression of c-Fos protein was detected after a single blue light pulse and depended on action potential firing, but not on synaptic activity. However, photocurrents diminished upon repeated stimulation and limited the number of action potential bursts that could be elicited.
conclusionsWe conclude that the C128A mutant is not suitable for chronic stimulation of neurons, but very useful for light-controlled induction of immediate early genes. This property of ChR2(C128A) could be harnessed to control the expression of proteins under control of the c-fos promoter with precise timing and single cell specificity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.