ArticlePloS one2009
Loss of the histone pre-mRNA processing factor stem-loop binding protein in Drosophila causes genomic instability and impaired cellular proliferation.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 20 citations in OpenAlex.
- Contribution of histone variants to aneuploidy: a cancer perspective.Frontiers in genetics · 2023Pooled it
- Downregulation of Stem-Loop Binding Protein by Nicotine via α7-Nicotinic Acetylcholine Receptor and Its Role in Nicotine-Induced Cell Transformation.Toxicological sciences : an official journal of the Society of Toxicology · 2022Article
- 53BP1-ACLY-SLBP-coordinated activation of replication-dependent histone biogenesis maintains genomic integrity.Nucleic acids research · 2022Article
- Stem-loop binding protein and metal carcinogenesis.Seminars in cancer biology · 2021Review
- Histone stress: an unexplored source of chromosomal instability in cancer?Current genetics · 2019Review
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- Article
- A subset of replication-dependent histone mRNAs are expressed as polyadenylated RNAs in terminally differentiated tissues.Nucleic acids research · 2016Article
- Arsenic induces polyadenylation of canonical histone mRNA by down-regulating stem-loop-binding protein gene expression.The Journal of biological chemistry · 2014Article
- Cyclin E2 induces genomic instability by mechanisms distinct from cyclin E1.Cell cycle (Georgetown, Tex.) · 2013Article
- R-loops and genomic instability in Bre1 (RNF20/40)-deficient cells.Cell cycle (Georgetown, Tex.) · 2012Article
- Ars2 promotes proper replication-dependent histone mRNA 3' end formation.Molecular cell · 2012Article
- Three structure-selective endonucleases are essential in the absence of BLM helicase in Drosophila.PLoS genetics · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMetazoan replication-dependent histone mRNAs terminate in a conserved stem-loop structure rather than a polyA tail. Formation of this unique mRNA 3' end requires Stem-loop Binding Protein (SLBP), which directly binds histone pre-mRNA and stimulates 3' end processing. The 3' end stem-loop is necessary for all aspects of histone mRNA metabolism, including replication coupling, but its importance to organism fitness and genome maintenance in vivo have not been characterized. METHODOLOGY/PRINCIPAL
findingsIn Drosophila, disruption of the Slbp gene prevents normal histone pre-mRNA processing and causes histone pre-mRNAs to utilize the canonical 3' end processing pathway, resulting in polyadenylated histone mRNAs that are no longer properly regulated. Here we show that Slbp mutants display genomic instability, including loss of heterozygosity (LOH), increased presence of chromosome breaks, tetraploidy, and changes in position effect variegation (PEV). During imaginal disc growth, Slbp mutant cells show defects in S phase and proliferate more slowly than control cells. CONCLUSIONS/SIGNIFICANCE: These data are consistent with a model in which changing the 3' end of histone mRNA disrupts normal replication-coupled histone mRNA biosynthesis and alters chromatin assembly, resulting in genomic instability, inhibition of cell proliferation, and impaired development.
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Registered trials
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