ArticleThe Journal of biological chemistry2010
Identification of human plasma proteins as major clients for the extracellular chaperone clusterin.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- Clusterin is enriched in plaque-associated astrocytes and localizes to synapses in Alzheimer's disease.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2026Article
- Revisiting Glomerulopathy in Clusterin Knockout Mice: A Mouse Model of Human Immunotactoid Glomerulopathy.Nephrology (Carlton, Vic.) · 2025Article
- Copper in Cancer: from transition metal to potential target.Human cell · 2024Review
- HDL Accessory Proteins in Parkinson's Disease-Focusing on Clusterin (Apolipoprotein J) in Regard to Its Involvement in Pathology and Diagnostics-A Review.Antioxidants (Basel, Switzerland) · 2022Review
- Plasma Proteomic Analysis in Morquio A Disease.International journal of molecular sciences · 2021Article
- Novel ACE2 protein interactions relevant to COVID-19 predicted by evolutionary rate correlations.PeerJ · 2021Article
- Clusterin Deficiency Predisposes C57BL/6j Mice to Cationic Bovine Serum Albumin-Induced Glomerular Inflammation.Journal of inflammation research · 2020Article
- Human pregnancy zone protein stabilizes misfolded proteins including preeclampsia- and Alzheimer's-associated amyloid beta peptide.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- How schistosomes alter the human serum proteome.Molecular and biochemical parasitology · 2017Article
- Altered levels of blood proteins in Alzheimer's disease longitudinal study: Results from Australian Imaging Biomarkers Lifestyle Study of Ageing cohort.Alzheimer's & dementia (Amsterdam, Netherlands) · 2017Article
- Cooperative stabilization of transthyretin by clusterin and diflunisal.Biochemistry · 2015Article
- LDL electronegativity index: a potential novel index for predicting cardiovascular disease.Vascular health and risk management · 2015Review
- Hypochlorite-induced structural modifications enhance the chaperone activity of human α2-macroglobulin.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Acute phase proteins are major clients for the chaperone action of α₂-macroglobulin in human plasma.Cell stress & chaperones · 2013Article
- Serum albumin prevents protein aggregation and amyloid formation and retains chaperone-like activity in the presence of physiological ligands.The Journal of biological chemistry · 2012Article
- Clusterin facilitates in vivo clearance of extracellular misfolded proteins.Cellular and molecular life sciences : CMLS · 2011Article
- Inhibition of beta2-microglobulin amyloid fibril formation by alpha2-macroglobulin.The Journal of biological chemistry · 2011Article
- Evidence for a functional role of the molecular chaperone clusterin in amyloidotic cardiomyopathy.The American journal of pathology · 2011Article
- Expression of metabolic, tissue remodeling, oxidative stress, and inflammatory pathways in mammary tissue during involution in lactating dairy cows.Bioinformatics and biology insights · 2010Article
- Levels of plasma ceruloplasmin protein are markedly lower following dietary copper deficiency in rodents.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clusterin (CLU) is an extracellular chaperone that is likely to play an important role in protein folding quality control. This study identified three deposition disease-associated proteins as major plasma clients for clusterin by studying CLU-client complexes formed in response to physiologically relevant stress (shear stress, approximately 36 dynes/cm(2) at 37 degrees C). Analysis of plasma samples by size exclusion chromatography indicated that (i) relative to control plasma, stressed plasma contained proportionally more soluble protein species of high molecular weight, and (ii) high molecular weight species were far more abundant when proteins purified by anti-CLU immunoaffinity chromatography from stressed plasma were compared with those purified from control plasma. SDS-PAGE and Western blot analyses indicated that a variety of proteins co-purified with CLU from both stressed and control plasma; however, several proteins were uniquely present or much more abundant when plasma was stressed. These proteins were identified by mass spectrometry as ceruloplasmin, fibrinogen, and albumin. Immunodot blot analysis of size exclusion chromatography fractionated plasma suggested that CLU-client complexes generated in situ are very large and may reach >or=4 x 10(7) Da. Lastly, sandwich enzyme-linked immunosorbent assay detected complexes containing CLU and ceruloplasmin, fibrinogen, or albumin in stressed but not control plasma. We have previously proposed that CLU-client complexes serve as vehicles to dispose of damaged misfolded extracellular proteins in vivo via receptor-mediated endocytosis. A better understanding of these mechanisms is likely to ultimately lead to the identification of new therapies for extracellular protein deposition disorders.
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Registered trials
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