ArticleThe Journal of biological chemistry2010
An alternative form of replication protein a expressed in normal human tissues supports DNA repair.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Altered memory CCR6EBioMedicine · 2024Article
- Review
- Replication Protein A, the Main Eukaryotic Single-Stranded DNA Binding Protein, a Focal Point in Cellular DNA Metabolism.International journal of molecular sciences · 2024Review
- Article
- Single-Stranded DNA-Binding Proteins in the Archaea.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Overlapping Structures Detection in Protein-Protein Interaction Networks Using Community Detection Algorithm Based on Neighbor Clustering Coefficient.Frontiers in genetics · 2021Article
- Dynamic elements of replication protein A at the crossroads of DNA replication, recombination, and repair.Critical reviews in biochemistry and molecular biology · 2020Review
- A nuclear-directed human pancreatic ribonuclease (PE5) targets the metabolic phenotype of cancer cells.Oncotarget · 2016Article
- Characterization of the transcriptional machinery bound across the widely presumed type 2 diabetes causal variant, rs7903146, within TCF7L2.European journal of human genetics : EJHG · 2015Article
- Global Identification of Genes Specific for Rice Meiosis.PloS one · 2015Article
- Identification of essential and non-essential single-stranded DNA-binding proteins in a model archaeal organism.Nucleic acids research · 2012Article
- The structure and function of replication protein A in DNA replication.Sub-cellular biochemistry · 2012Review
- The DNA damage response kinases DNA-dependent protein kinase (DNA-PK) and ataxia telangiectasia mutated (ATM) Are stimulated by bulky adduct-containing DNA.The Journal of biological chemistry · 2011Article
- Multiple ATR-Chk1 pathway proteins preferentially associate with checkpoint-inducing DNA substrates.PloS one · 2011Article
- ATRIP from TopBP1 to ATR--in vitro activation of a DNA damage checkpoint.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Reconstitution of RPA-covered single-stranded DNA-activated ATR-Chk1 signaling.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Oligonucleotide/oligosaccharide-binding fold proteins: a growing family of genome guardians.Critical reviews in biochemistry and molecular biology · 2010Review
- Functions of alternative replication protein A in initiation and elongation.Biochemistry · 2010Article
- Tipin-replication protein A interaction mediates Chk1 phosphorylation by ATR in response to genotoxic stress.The Journal of biological chemistry · 2010Article
- Genome Stability Maintenance in Naked Mole-Rat.Acta naturaeArticle
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
Abstract
Replication protein A (RPA) is a heterotrimeric protein complex required for a large number of DNA metabolic processes, including DNA replication and repair. An alternative form of RPA (aRPA) has been described in which the RPA2 subunit (the 32-kDa subunit of RPA and product of the RPA2 gene) of canonical RPA is replaced by a homologous subunit, RPA4. The normal function of aRPA is not known; however, previous studies have shown that it does not support DNA replication in vitro or S-phase progression in vivo. In this work, we show that the RPA4 gene is expressed in normal human tissues and that its expression is decreased in cancerous tissues. To determine whether aRPA plays a role in cellular physiology, we investigated its role in DNA repair. aRPA interacted with both Rad52 and Rad51 and stimulated Rad51 strand exchange. We also showed that, by using a reconstituted reaction, aRPA can support the dual incision/excision reaction of nucleotide excision repair. aRPA is less efficient in nucleotide excision repair than canonical RPA, showing reduced interactions with the repair factor XPA and no stimulation of XPF-ERCC1 endonuclease activity. In contrast, aRPA exhibits higher affinity for damaged DNA than canonical RPA, which may explain its ability to substitute for RPA in the excision step of nucleotide excision repair. Our findings provide the first direct evidence for the function of aRPA in human DNA metabolism and support a model for aRPA functioning in chromosome maintenance functions in nonproliferating cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.