ArticleBlood2010
Role for ADAP in shear flow-induced platelet mechanotransduction.
Article in Blood, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- Temporal phosphoproteomics reveals key regulators of procoagulant COAT platelet generation.Blood vessels, thrombosis & hemostasis · 2026Article
- Emerging Elastic Micro-Nano Materials for Diagnosis and Treatment of Thrombosis.Research (Washington, D.C.) · 2025Review
- Structure, signal transduction, activation, and inhibition of integrin αIIbβ3.Thrombosis journal · 2023Review
- Platelet SHARPIN regulates platelet adhesion and inflammatory responses through associations with αIIbβ3 and LUBAC.Blood advances · 2022Article
- Characterization of Mice with a Platelet-Specific Deletion of the Adapter Molecule ADAP.Molecular and cellular biology · 2019Article
- Platelet integrin αIIbβ3: signal transduction, regulation, and its therapeutic targeting.Journal of hematology & oncology · 2019Review
- Immune Cell-Type Specific Ablation of Adapter Protein ADAP Differentially Modulates EAE.Frontiers in immunology · 2019Article
- Integrin αBlood · 2017Review
- Metastasis of circulating tumor cells: favorable soil or suitable biomechanics, or both?Cell adhesion & migration · 2015Review
- Recessive thrombocytopenia likely due to a homozygous pathogenic variant in the FYB gene: case report.BMC medical genetics · 2014Article
- Article
- Dok-2 adaptor protein regulates the shear-dependent adhesive function of platelet integrin αIIbβ3 in mice.The Journal of biological chemistry · 2014Article
- Shear Stress Enhances Chemokine Secretion fromCellular and molecular bioengineering · 2013Article
- Platelet transport rates and binding kinetics at high shear over a thrombus.Biophysical journal · 2013Article
- Biomechanics of haemostasis and thrombosis in health and disease: from the macro- to molecular scale.Journal of cellular and molecular medicine · 2013Review
- A role for adhesion and degranulation-promoting adapter protein in collagen-induced platelet activation mediated via integrin α(2) β(1).Journal of thrombosis and haemostasis : JTH · 2012Article
- Mapping the mechanome of live stem cells using a novel method to measure local strain fields in situ at the fluid-cell interface.PloS one · 2012Article
- The insider's guide to leukocyte integrin signalling and function.Nature reviews. Immunology · 2011Review
- Platelet receptor signaling in thrombus formation.Journal of molecular medicine (Berlin, Germany) · 2011Review
- Hydrodynamic regulation of monocyte inflammatory response to an intracellular pathogen.PloS one · 2011Article
Corrections and comments
- Commented on by
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Binding of platelets to fibrinogen via integrin alphaIIbbeta3 stimulates cytoskeletal reorganization and spreading. These responses depend on tyrosine phosphorylation of multiple proteins by Src family members and Syk. Among Src substrates in platelets is adhesion- and degranulation-promoting adapter protein (ADAP), an adapter with potential binding partners: SLP-76, VASP, and SKAP-HOM. During studies of platelet function under shear flow, we discovered that ADAP(-/-) mouse platelets, unlike ADAP+/+ platelets, formed unstable thrombi in response to carotid artery injury. Moreover, fibrinogen-adherent ADAP(-/-) platelets in shear flow ex vivo showed reduced spreading and smaller zones of contact with the matrix. These abnormalities were not observed under static conditions, and they could not be rescued by stimulating platelets with a PAR4 receptor agonist or by direct alphaIIbbeta3 activation with MnCl2, consistent with a defect in outside-in alphaIIbbeta3 signaling. ADAP+/+ platelets subjected to shear flow assembled F-actin-rich structures that colocalized with SLP-76 and the Rac1 exchange factor, phospho-Vav1. In contrast, platelets deficient in ADAP, but not those deficient in VASP or SKAP-HOM, failed to form these structures. These results establish that ADAP is an essential component of alphaIIbbeta3-mediated platelet mechanotransduction that promotes F-actin assembly and enables platelet spreading and thrombus stabilization under fluid shear stress.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.