ArticleProceedings of the National Academy of Sciences of the United States of America2009
Nickel-inducible lysis system in Synechocystis sp. PCC 6803.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 96 citations in OpenAlex.
- Co-expression of holin gene improves heterologous cellulase secretion and surface display by lactic acid bacteria Lactococcus cremoris.Journal of biological engineering · 2026Article
- Engineering bacteria for enhanced tumor therapy: from surface modification to synthetic genetic circuits.Journal of hematology & oncology · 2025Review
- Engineering Dual-Input Glucose- and Temperature-Sensitive Lysis Circuits inMicroorganisms · 2025Article
- Engineering of bacteria towards programmed autolysis: why, how, and when?Microbial cell factories · 2024Review
- Polyphosphate kinase deletion increases laboratory productivity in cyanobacteria.Frontiers in plant science · 2024Article
- Incorporation, fate, and turnover of free fatty acids in cyanobacteria.FEMS microbiology reviews · 2023Review
- Biocontainment of Genetically Engineered Algae.Frontiers in plant science · 2022Review
- Bacteriophage-encoded lethal membrane disruptors: Advances in understanding and potential applications.Frontiers in microbiology · 2022Review
- Article
- Functional Analysis of the Endopeptidase and Holin FromFrontiers in microbiology · 2022Article
- Developing algae as a sustainable food source.Frontiers in nutrition · 2022Review
- Decoding the molecular properties of mycobacteriophage D29 Holin provides insights into Holin engineering.Journal of virology · 2021Article
- A Green Light-Regulated T7 RNA Polymerase Gene Expression System for Cyanobacteria.Marine biotechnology (New York, N.Y.) · 2021Article
- Engineering the Osmotic State ofFrontiers in bioengineering and biotechnology · 2020Article
- Characterizing active transportation mechanisms for free fatty acids and antibiotics in Synechocystis sp. PCC 6803.BMC biotechnology · 2019Article
- Improved lipid production via fatty acid biosynthesis and free fatty acid recycling in engineeredBiotechnology for biofuels · 2019Article
- The Development of an Effective Bacterial Single-Cell Lysis Method Suitable for Whole Genome Amplification in Microfluidic Platforms.Micromachines · 2018Article
- A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli.Bioengineered · 2017Article
- A novel programmable lysozyme-based lysis system in Pseudomonas putida for biopolymer production.Scientific reports · 2017Article
- Engineering Cyanobacterial Cell Morphology for Enhanced Recovery and Processing of Biomass.Applied and environmental microbiology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We designed and constructed a controllable inducing lysis system in Synechocystis sp. PCC 6803 to facilitate extracting lipids for biofuel production. Several bacteriophage-derived lysis genes were integrated into the genome and placed downstream of a nickel-inducible signal transduction system. We applied 3 strategies: (i) directly using the phage lysis cassette, (ii) constitutively expressing endolysin genes while restricting holin genes, and (iii) combining lysis genes from different phages. Significant autolysis was induced in the Synechocystis sp. PCC 6803 cells with this system by the addition of NiSO(4). Our inducible cyanobacterial lysing system eliminates the need for mechanical or chemical cell breakage and could facilitate recovery of biofuel from cyanobacteria.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.