ArticleMolecular and cellular biology2010
Selective signaling by Akt2 promotes bone morphogenetic protein 2-mediated osteoblast differentiation.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 75 citations in OpenAlex.
- Pannexins in the musculoskeletal system: new targets for development and disease progression.Bone research · 2024Review
- Phenytoin regulates osteogenic differentiation of human bone marrow stem cells by PI3K/Akt pathway.Regenerative therapy · 2023Article
- Protein tyrosine phosphatases in skeletal development and diseases.Bone research · 2022Review
- Calycosin-7-O-β-Glucoside Isolated fromInternational journal of molecular sciences · 2021Article
- Integrated analysis of miRNA and mRNA transcriptomic reveals antler growth regulatory network.Molecular genetics and genomics : MGG · 2021Article
- Critical implication of the PTEN/PI3K/AKT pathway during BMP2-induced heterotopic ossification.Molecular medicine reports · 2021Article
- Wrong place, wrong time: Runt-related transcription factor 2/SATB2 pathway in bone development and carcinogenesis.Journal of carcinogenesis · 2021Review
- Microarray analysis identification of key pathways and interaction network of differential gene expressions during osteogenic differentiation.Human genomics · 2020Article
- p75NTRCell proliferation · 2020Article
- Plastrum Testudinis Extract Mitigates Thiram Toxicity in Broilers via Regulating PI3K/AKT Signaling.Biomolecules · 2019Article
- Phloretin Suppresses Bone Morphogenetic Protein-2-Induced Osteoblastogenesis and Mineralization via Inhibition of Phosphatidylinositol 3-kinases/Akt Pathway.International journal of molecular sciences · 2019Article
- Biofunctional soyasaponin Bb in peanut (Arachis hypogaea L.) sprouts enhances bone morphogenetic protein-2-dependent osteogenic differentiation via activation of runt-related transcription factor 2 in C2C12 cells.Phytotherapy research : PTR · 2019Article
- A PDGFRβ-PI3K signaling axis mediates periosteal cell activation during fracture healing.PloS one · 2019Article
- KR-12-a5 Reverses Adverse Effects of Lipopolysaccharides on HBMSC Osteogenic Differentiation by Influencing BMP/Smad and P38 MAPK Signaling Pathways.Frontiers in pharmacology · 2019Article
- Identification of miR-708-5p in peripheral blood monocytes: Potential marker for postmenopausal osteoporosis in Mexican-Mestizo population.Experimental biology and medicine (Maywood, N.J.) · 2018Article
- Insights into inflammatory priming of mesenchymal stromal cells: functional biological impacts.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2018Review
- The antimicrobial peptide KR-12 promotes the osteogenic differentiation of human bone marrow stem cells by stimulating BMP/SMAD signaling.RSC advances · 2018Article
- Synergistic Effects of Controlled-Released BMP-2 and VEGF from nHAC/PLGAs Scaffold on Osteogenesis.BioMed research international · 2018Article
- AKT2 is involved in the IL‑17A‑mediated promotion of differentiation and calcification of murine preosteoblastic MC3T3‑E1 cells.Molecular medicine reports · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
Mesenchymal stem cells are essential for repair of bone and other supporting tissues. Bone morphogenetic proteins (BMPs) promote commitment of these progenitors toward an osteoblast fate via functional interactions with osteogenic transcription factors, including Dlx3, Dlx5, and Runx2, and also can direct their differentiation into bone-forming cells. BMP-2-stimulated osteoblast differentiation additionally requires continual signaling from insulin-like growth factor (IGF)-activated pathways. Here we identify Akt2 as a critical mediator of IGF-regulated osteogenesis. Targeted knockdown of Akt2 in mouse primary bone marrow stromal cells or in a mesenchymal stem cell line, or genetic knockout of Akt2, did not interfere with BMP-2-mediated signaling but resulted in inhibition of osteoblast differentiation at an early step that preceded production of Runx2. In contrast, Akt1-deficient cells differentiated normally. Complete biochemical and morphological osteoblast differentiation was restored in cells lacking Akt2 by adenoviral delivery of Runx2 or by a recombinant lentivirus encoding wild-type Akt2. In contrast, lentiviral Akt1 was ineffective. Taken together, these observations define a specific role for Akt2 as a gatekeeper of osteogenic differentiation through regulation of Runx2 gene expression and indicate that the closely related Akt1 and Akt2 exert distinct effects on the differentiation of mesenchymal precursors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.