ArticleBMC genomics2009
Identification of expressed genes during compatible interaction between stripe rust (Puccinia striiformis) and wheat using a cDNA library.
Article in BMC genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 60 citations in OpenAlex.
- CRISPR-mediated genome editing of wheat for enhancing disease resistance.Frontiers in genome editing · 2025Review
- Review
- Functional Characterization of the Wheat Macrophage Migration Inhibitory Factor TaMIF1 in Wheat-Stripe Rust (Biology · 2021Article
- Article
- Article
- Ethylene modulates root cortical senescence in barley.Annals of botany · 2018Article
- Wheat GeneInternational journal of molecular sciences · 2018Article
- Host-induced gene silencing of an important pathogenicity factor PsCPK1 in Puccinia striiformis f. sp. tritici enhances resistance of wheat to stripe rust.Plant biotechnology journal · 2018Article
- TaARPC3, Contributes to Wheat Resistance against the Stripe Rust Fungus.Frontiers in plant science · 2017Article
- Cellular and molecular characterization of a stem rust resistance locus on wheat chromosome 7AL.BMC research notes · 2016Article
- Characterization of a Ran gene from Puccinia striiformis f. sp. tritici involved in fungal growth and anti-cell death.Scientific reports · 2016Article
- TaTypA, a Ribosome-Binding GTPase Protein, Positively Regulates Wheat Resistance to the Stripe Rust Fungus.Frontiers in plant science · 2016Article
- PsANT, the adenine nucleotide translocase of Puccinia striiformis, promotes cell death and fungal growth.Scientific reports · 2015Article
- Construction of a High-Quality Yeast Two-Hybrid Library and Its Application in Identification of Interacting Proteins with Brn1 in Curvularia lunata.The plant pathology journal · 2015Article
- Wheat stripe (yellow) rust caused by Puccinia striiformis f. sp. tritici.Molecular plant pathology · 2014Review
- Construction and identification of a cDNA library for use in the yeast two-hybrid system from duck embryonic fibroblast cells post-infected with duck enteritis virus.Molecular biology reports · 2014Article
- TaEIL1, a wheat homologue of AtEIN3, acts as a negative regulator in the wheat-stripe rust fungus interaction.Molecular plant pathology · 2013Article
- Identification of Lens culinaris defense genes responsive to the anthracnose pathogen Colletotrichum truncatum.BMC genetics · 2013Article
- Article
- Strategies for Wheat Stripe Rust Pathogenicity Identified by Transcriptome Sequencing.PloS one · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat worldwide. To establish compatibility with the host, Pst forms special infection structures to invade the plant with minimal damage to host cells. Although compatible interaction between wheat and Pst has been studied using various approaches, research on molecular mechanisms of the interaction is limited. The aim of this study was to develop an EST database of wheat infected by Pst in order to determine transcription profiles of genes involved in compatible wheat-Pst interaction.
resultsTotal RNA, extracted from susceptible infected wheat leaves harvested at 3, 5 and 8 days post inoculation (dpi), was used to create a cDNA library, from which 5,793 ESTs with high quality were obtained and clustered into 583 contigs and 2,160 singletons to give a set of 2,743 unisequences (GenBank accessions: GR302385 to GR305127). The BLASTx program was used to search for homologous genes of the unisequences in the GenBank non-redundant protein database. Of the 2,743 unisequences, 52.8% (the largest category) were highly homologous to plant genes; 16.3% to fungal genes and 30% of no-hit. The functional classification of all ESTs was established based on the database entry giving the best E-value using the Bevan's classification categories. About 50% of the ESTs were significantly homologous to genes encoding proteins with known functions; 20% were similar to genes encoding proteins with unknown functions and 30% did not have significant homology to any sequence in the database. The quantitative real-time PCR (qRT-PCR) analysis determined the transcription profiles and their involvement in the wheat-Pst interaction for seven of the gene.
conclusionThe cDNA library is useful for identifying the functional genes involved in the wheat-Pst compatible interaction, and established a new database for studying Pst pathogenesis genes and wheat defense genes. The transcription patterns of seven genes were confirmed by the qRT-PCR assay to be differentially expressed in wheat-Pst compatible and incompatible interaction.
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