ArticleTheoretical biology & medical modelling2009
In silico analysis of chimeric espA, eae and tir fragments of Escherichia coli O157:H7 for oral immunogenic applications.
Article in Theoretical biology & medical modelling, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- Towards scalable production of recombinant EIT as a protective antigen against EHEC.Bioprocess and biosystems engineering · 2026Article
- Developing a simple, cost-effective proof-of-concept vaccine candidate against EHEC for cattle.PloS one · 2026Article
- Oral Administration with Live Attenuated Citrobacter rodentium Protects Immunocompromised Mice from Lethal Infection.Infection and immunity · 2022Article
- A novel B- and helper T-cell epitopes-based prophylactic vaccine againstBioImpacts : BI · 2018Article
- Immunogenic evaluation of chimeric recombinant proteinMolecular biology research communications · 2017Article
- Expression and purification of recombinant HTLV-I/-II linear epitopes antigen and its application for screening of suspected patients.Iranian journal of microbiology · 2017Article
- In silico designing, cloning, and heterologous expression of novel chimeric human B lymphocyte CD20 extra loop.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- In silico analysis of an envelope domain III-based multivalent fusion protein as a potential dengue vaccine candidate.Clinical and experimental vaccine research · 2016Article
- In Silico Design of a Chimeric Protein Containing Antigenic Fragments of Helicobacter pylori; A Bioinformatic Approach.The open microbiology journal · 2016Article
- Chitosan-Based Intranasal Vaccine against Escherichia coli O157:H7.Iranian biomedical journal · 2016Article
- Heterologous Expression of Hepatitis C Virus Core Protein in Oil Seeds of Brassica napus L.Jundishapur journal of microbiology · 2015Article
- Immune reactivity of sera obtained from brucellosis patients and vaccinated-rabbits to a fusion protein from Brucella melitensis.Iranian journal of basic medical sciences · 2015Article
- In silico analysis for identifying potential vaccine candidates against Staphylococcus aureus.Clinical and experimental vaccine research · 2015Article
- Enhanced-Transient Expression of Hepatitis C Virus Core Protein in Nicotiana tabacum, a Protein With Potential Clinical Applications.Hepatitis monthly · 2014Article
- Designing and analyzing the structure of Tat-BoNT/A(1-448) fusion protein: AnMolecular biology research communications · 2014Article
- Recent advances on host plants and expression cassettes' structure and function in plant molecular pharming.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2014Review
- In silico analysis of chimeric TF, Omp31 and BP26 fragments of Brucella melitensis for development of a multi subunit vaccine candidate.Iranian journal of basic medical sciences · 2014Article
- In Silico Design and Analysis of TGFαL3-SEB Fusion Protein as "a New Antitumor Agent" Candidate by Ligand-Targeted Superantigens Technique.Iranian journal of cancer prevention · 2014Article
- Immunization with H7-HCP-tir-intimin significantly reduces colonization and shedding of Escherichia coli O157:H7 in goats.PloS one · 2014Article
- EspA-Intimin chimeric protein, a candidate vaccine against Escherichia coli O157:H7.Iranian journal of microbiology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn silico techniques are highly suited for both the discovery of new and development of existing vaccines. Enterohemorrhagic Escherichia coli O157:H7 (EHEC) exhibits a pattern of localized adherence to host cells, with the formation of microcolonies, and induces a specific histopathological lesion (attaching/effacing). The genes encoding the products responsible for this phenotype are clustered on a 35-kb pathogenicity island. Among these proteins, Intimin, Tir, and EspA, which are expressed by attaching-effacing genes, are responsible for the attachment to epithelial cell that leads to lesions.
resultsWe designed synthetic genes encoding the carboxy-terminal fragment of Intimin, the middle region of Tir and the carboxy-terminal part of EspA. These multi genes were synthesized with codon optimization for a plant host and were fused together by the application of four repeats of five hydrophobic amino acids as linkers. The structure of the synthetic construct gene, its mRNA and deduced protein and their stabilities were analyzed by bioinformatic software. Furthermore, the immunogenicity of this multimeric recombinant protein consisting of three different domains was predicted.
conclusiona structural model for a chimeric gene from LEE antigenic determinants of EHEC is presented. It may define accessibility, solubility and immunogenecity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.