ArticleJournal of proteome research2010
Association of immunosuppressant-induced protein changes in the rat kidney with changes in urine metabolite patterns: a proteo-metabonomic study.
Article in Journal of proteome research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- Chronic dehydration induces injury pathways in rats, but does not mimic histopathology of chronic interstitial nephritis in agricultural communities.Scientific reports · 2023Article
- Targeted Urine Metabolomics for Monitoring Renal Allograft Injury and Immunosuppression in Pediatric Patients.Journal of clinical medicine · 2020Article
- Identification of Novel Biomarkers for Predicting Kidney Injury Due to Drugs Using "Omic" Strategies.Pharmacogenomics and personalized medicine · 2020Review
- Everolimus and sirolimus in transplantation-related but different.Expert opinion on drug safety · 2015Review
- A high-performance liquid chromatography-tandem mass spectrometry-based targeted metabolomics kidney dysfunction marker panel in human urine.Clinica chimica acta; international journal of clinical chemistry · 2015Article
- Regucalcin as a potential biomarker for metabolic and neuronal diseases.Molecular and cellular biochemistry · 2014Review
- Mycophenolate mofetil enhances the negative effects of sirolimus and tacrolimus on rat kidney cell metabolism.PloS one · 2014Article
- Effects of immunosuppressive treatment on protein expression in rat kidney.Drug design, development and therapy · 2014Article
- Serum aminoacylase-1 is a novel biomarker with potential prognostic utility for long-term outcome in patients with delayed graft function following renal transplantation.Kidney international · 2013Article
- High-Throughput Proteomic Approaches to the Elucidation of Potential Biomarkers of Chronic Allograft Injury (CAI).Proteomes · 2013Review
- Proteomics and metabolomics in renal transplantation-quo vadis?Transplant international : official journal of the European Society for Organ Transplantation · 2013Review
- Low-salt diet and cyclosporine nephrotoxicity: changes in kidney cell metabolism.Journal of proteome research · 2012Article
- Everolimus and sirolimus in combination with cyclosporine have different effects on renal metabolism in the rat.PloS one · 2012Article
- Biomarkers of immunosuppressant organ toxicity after transplantation: status, concepts and misconceptions.Expert opinion on drug metabolism & toxicology · 2011Review
- Biomarkers of acute kidney injury.Journal of toxicology · 2011Article
- Toxicodynamic effects of ciclosporin are reflected by metabolite profiles in the urine of healthy individuals after a single dose.British journal of clinical pharmacology · 2010Article
- Effects of an immunosuppressive treatment on the rat prostate.Drug design, development and therapyArticle
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The basic mechanisms underlying calcineurin inhibitor (CI) nephrotoxicity and its enhancement by sirolimus are still largely unknown. We investigated the effects of CIs alone and in combination with sirolimus on the renal proteome and correlated these effects with urine metabolite pattern changes. Thirty-six male Wistar rats were assigned to six treatment groups (n = 4/group for proteome analysis and n = 6/group for urine (1)H NMR metabolite pattern analysis): vehicle controls, sirolimus 1 mg/kg/day, cyclosporine 10 mg/kg/day, cyclosporine 10 mg/kg/day + sirolimus 1 mg/kg/day, tacrolimus 1 mg/kg/day, tacrolimus 1 mg/kg/day + sirolimus 1 mg/kg/day. After 28 days, 24 h-urine was collected for (1)H NMR-based metabolic analysis and kidneys were harvested for 2D-gel electrophoresis and histology. Cyclosporine affected the following groups of proteins: calcium homeostasis (regucalcin, calbindin), cytoskeleton (vimentin, caldesmon), response to hypoxia and mitochondrial function (prolyl 4-hydroxylase, proteasome, NADH dehydrogenase), and cell metabolism (kidney aminoacylase, pyruvate dehydrogenase, fructose-1,6-bis phosphate). Several of the changes in protein expression, confirmed by Western blot, were associated with and explained changes in metabolite concentrations in urine. Representative examples are an increase in kidney aminoacylase expression (decrease of hippurate concentrations in urine), up regulation of pyruvate dehydrogenase and fructose-1,6-bisphosphatase, (increased glucose metabolism), and down regulation of arginine/glycine-amidino transferase (most likely due to an increase in creatinine concentrations). Protein changes explained and qualified immunosuppressant-induced metabolite pattern changes in urine.
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Registered trials
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