ArticleInfection and immunity1991
Effects of cytochalasin D and methylamine on intracellular growth of Legionella pneumophila in amoebae and human monocyte-like cells.
Article in Infection and immunity, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed, 91 citations in OpenAlex.
- Ecology ofBiofilm · 2024Review
- Neutrophil heterogeneity in complement C1q expression associated with sepsis mortality.Frontiers in immunology · 2022Article
- Pathogenicity and Virulence ofVirulence · 2021Review
- Review
- From Many Hosts, One Accidental Pathogen: The Diverse Protozoan Hosts ofFrontiers in cellular and infection microbiology · 2017Review
- Identification of regions within the Legionella pneumophila VipA effector protein involved in actin binding and polymerization and in interference with eukaryotic organelle trafficking.MicrobiologyOpen · 2016Article
- Mechanisms associated with phagocytosis of Arcobacter butzleri by Acanthamoeba castellanii.Parasitology research · 2014Article
- Mechanism involved in phagocytosis and killing of Listeria monocytogenes by Acanthamoeba polyphaga.Parasitology research · 2009Article
- Article
- Extraintestinal pathogenic Escherichia coli survives within neutrophils.Infection and immunity · 2007Article
- Impact of non-Legionella bacteria on the uptake and intracellular replication of Legionella pneumophila in Acanthamoeba castellanii and Naegleria lovaniensis.Microbial ecology · 2005Article
- Interaction of Pasteurella multocida with free-living amoebae.Applied and environmental microbiology · 2005Article
- Balamuthia mandrillaris, free-living ameba and opportunistic agent of encephalitis, is a potential host for Legionella pneumophila bacteria.Applied and environmental microbiology · 2005Article
- Microorganisms resistant to free-living amoebae.Clinical microbiology reviews · 2004Review
- Acanthamoeba spp. as agents of disease in humans.Clinical microbiology reviews · 2003Review
- Legionella and Legionnaires' disease: 25 years of investigation.Clinical microbiology reviews · 2002Review
- Flagellum of Legionella pneumophila positively affects the early phase of infection of eukaryotic host cells.Infection and immunity · 2001Article
- Identification of Legionella pneumophila genes important for infection of amoebas by signature-tagged mutagenesis.Infection and immunity · 2001Article
- Legionella pneumophila replication vacuoles mature into acidic, endocytic organelles.The Journal of experimental medicine · 2000Article
- Intracellular growth in Acanthamoeba castellanii affects monocyte entry mechanisms and enhances virulence of Legionella pneumophila.Infection and immunity · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cloned and axenically cultured strain of Hartmannella vermiformis was used as a model to study intracellular multiplication of Legionella pneumophila in amoebae. The growth of L. pneumophilia in both H. vermiformis and a human monocyte-like cell line (U937) was investigated with cytoskeletal and metabolic inhibitors. L. pneumophila replicated only intracellularly in these cellular models, and electron microscopy showed ultrastructural similarities in the initial phase of multiplication. Treatment of amoebae with an inhibitor of microfilament-dependent phagocytosis (cytochalasin D, 0.5 or 1.0 micrograms/ml) did not inhibit intracellular growth of L. pneumophila; however, intracellular multiplication was inhibited by treatment of U937 monocytes with the same concentrations of cytochalasin D. Methylamine (10 to 100 mM), an inhibitor of adsorptive pinocytosis, inhibited the replication of L. pneumophila in amoebae in a dose-dependent manner. All doses of methylamine tested (10 to 50 mM) inhibited growth of L. pneumophila in U937 monocytes. Cytochalasin D and methylamine had no effect on the multiplication of L. pneumophila in culture medium or on the viability of amoebae or U937 monocytes. Intracellular replication of L. pneumophila in H. vermiformis may be accomplished by a cytochalasin D-independent mechanism, such as adsorptive pinocytosis. In contrast, both cytochalasin D- and methylamine-sensitive mechanisms may be essential for the intracellular multiplication of L. pneumophila in U937 monocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.