ArticleThe Biochemical journal1991
A soluble 5'-nucleotidase in rat kidney. Stimulation by decavanadate.
Article in The Biochemical journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 16 citations in OpenAlex.
- Bioactivity of Decavanadate Compounds: Can TheirInorganic chemistry · 2025Article
- Allosteric regulation and substrate activation in cytosolic nucleotidase II from Legionella pneumophila.The FEBS journal · 2014Article
- Reconstitution of 5'-nucleotidase of bull seminal plasma in spin-labeled liposomes.The Journal of membrane biology · 1994Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
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Abstract
A soluble 5'-nucleotidase was identified in rat kidney and partially purified. Compared with 5'-IMP, 5'-AMP was a poor substrate. The affinity for 5'-IMP was very low (S0.5 greater than 1 mM) in the absence of an activator, and it was much increased (S0.5 = 0.1 mM) by 2,3-bisphosphoglycerate (2,3-DPG). ATP and bisadenosyl tetraphosphate were further activators. The pH optimum was 6.3. Those properties suggest that the renal soluble 5'-nucleotidase is identical with the 'high-Km' 5'-nucleotidase purified previously from liver, heart and erythrocytes. Decavanadate (100 nM) increased the rate of hydrolysis of 1 mM-5'-IMP 16-fold. The effect was specific for the decameric form of vanadate, since it was not reproduced by either decavanadate-free orthovanadate or pervanadate. Half-maximal activation was obtained at 1.4 nM-decavanadate. Decavanadate increased the affinity of the soluble 5'-nucleotidase for 5'-IMP. The effects of 2,3-DPG and of vanadate were not additive. Thus decavanadate probably influences the soluble 5'-nucleotidase in the same way as 2,3-DPG, but with a much higher potency.
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