ArticleNucleic acids research2010
Chain and conformation stability of solid-state DNA: implications for room temperature storage.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 167 citations in OpenAlex.
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- Predict the degree of secondary structures of the encoding sequences in DNA storage by deep learning model.Scientific reports · 2025Article
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- Quantification of fungal biomass in mycelium composites made from diverse biogenic side streams.Fungal biology and biotechnology · 2024Article
- Efficient and low-complexity variable-to-variable length coding for DNA storage.BMC bioinformatics · 2024Article
- Recent progress in DNA data storage based on high-throughput DNA synthesis.Biomedical engineering letters · 2024Review
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- A Novel Image Encryption Scheme for DNA Storage Systems Based on DNA Hybridization and Gene Mutation.Interdisciplinary sciences, computational life sciences · 2023Article
- Dibenzo[International journal of molecular sciences · 2023Review
- DNA Data Storage.Biotech (Basel (Switzerland)) · 2023Review
- Passive collection of ticks in New Hampshire reveals species-specific patterns of distribution and activity.Journal of medical entomology · 2023Article
- Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage.Science advances · 2023Article
- Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage.Frontiers in veterinary science · 2023Article
- Towards Chinese text and DNA shift encoding scheme based on biomass plasmid storage.Frontiers in bioinformatics · 2023Article
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- Information decay and enzymatic information recovery for DNA data storage.Communications biology · 2022Article
- Design considerations for advancing data storage with synthetic DNA for long-term archiving.Materials today. Bio · 2022Review
Corrections and comments
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Authors and funding
7 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is currently wide interest in room temperature storage of dehydrated DNA. However, there is insufficient knowledge about its chemical and structural stability. Here, we show that solid-state DNA degradation is greatly affected by atmospheric water and oxygen at room temperature. In these conditions DNA can even be lost by aggregation. These are major concerns since laboratory plastic ware is not airtight. Chain-breaking rates measured between 70 degrees C and 140 degrees C seemed to follow Arrhenius' law. Extrapolation to 25 degrees C gave a degradation rate of about 1-40 cuts/10(5) nucleotides/century. However, these figures are to be taken as very tentative since they depend on the validity of the extrapolation and the positive or negative effect of contaminants, buffers or additives. Regarding the secondary structure, denaturation experiments showed that DNA secondary structure could be preserved or fully restored upon rehydration, except possibly for small fragments. Indeed, below about 500 bp, DNA fragments underwent a very slow evolution (almost suppressed in the presence of trehalose) which could end in an irreversible denaturation. Thus, this work validates using room temperature for storage of DNA if completely protected from water and oxygen.
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