ArticleJournal of molecular biology2010
Single-molecule study of DNA polymerization activity of HIV-1 reverse transcriptase on DNA templates.
Article in Journal of molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Reverse transcription of plasma-derived HIV-1 RNA generates multiple artifacts through tRNA(Lys-3)-priming.Microbiology spectrum · 2024Article
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- Zipper head mechanism of telomere synthesis by human telomerase.Cell research · 2021Article
- Replicative DNA polymerases promote active displacement of SSB proteins during lagging strand synthesis.Nucleic acids research · 2019Article
- The mechano-chemistry of a monomeric reverse transcriptase.Nucleic acids research · 2017Article
- Diatrack particle tracking software: Review of applications and performance evaluation.Traffic (Copenhagen, Denmark) · 2017Review
- Pausing kinetics dominates strand-displacement polymerization by reverse transcriptase.Nucleic acids research · 2017Article
- Regulation of yeast DNA polymerase δ-mediated strand displacement synthesis by 5'-flaps.Nucleic acids research · 2015Article
- Inventory of telomerase components in human cells reveals multiple subpopulations of hTR and hTERT.Nucleic acids research · 2014Article
- Biophysical Insights into the Inhibitory Mechanism of Non-Nucleoside HIV-1 Reverse Transcriptase Inhibitors.Biomolecules · 2013Article
- Examining the role of the HIV-1 reverse transcriptase p51 subunit in positioning and hydrolysis of RNA/DNA hybrids.The Journal of biological chemistry · 2013Article
- Analysis of RNA base modification and structural rearrangement by single-molecule real-time detection of reverse transcription.Journal of nanobiotechnology · 2013Article
- Mechanism of strand displacement synthesis by DNA replicative polymerases.Nucleic acids research · 2012Article
- Collaborative coupling between polymerase and helicase for leading-strand synthesis.Nucleic acids research · 2012Article
- Active DNA unwinding dynamics during processive DNA replication.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Minimalist model for force-dependent DNA replication.Biophysical journal · 2012Article
- Article
- Deep sequencing analysis of mutations resulting from the incorporation of dNTP analogs.Nucleic acids research · 2010Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
HIV-1 RT (human immunodeficiency virus-1 reverse transcriptase) is a multifunctional polymerase responsible for reverse transcription of the HIV genome, including DNA replication on both RNA and DNA templates. During reverse transcription in vivo, HIV-1 RT replicates through various secondary structures on RNA and single-stranded DNA (ssDNA) templates without the need for a nucleic acid unwinding protein, such as a helicase. In order to understand the mechanism of polymerization through secondary structures, we investigated the DNA polymerization activity of HIV-1 RT on long ssDNA templates using a multiplexed single-molecule DNA flow-stretching assay. We observed that HIV-1 RT performs fast primer extension DNA synthesis on single-stranded regions of DNA (18.7 nt/s) and switches its activity to slow strand displacement synthesis at DNA hairpin locations (2.3 nt/s). Furthermore, we found that the rate of strand displacement synthesis is dependent on the GC content in hairpin stems and template stretching force. This indicates that the strand displacement synthesis occurs through a mechanism that is neither completely active nor passive: that is, the opening of the DNA hairpin is driven by a combination of free energy released during dNTP (deoxyribonucleotide triphosphate) hydrolysis and thermal fraying of base pairs. Our experimental observations provide new insight into the interchanging modes of DNA replication by HIV-1 RT on long ssDNA templates.
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Registered trials
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