ArticleBioconjugate chemistry2010
Synthesis and in vitro testing of new potent polyacridine-melittin gene delivery peptides.
Article in Bioconjugate chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 43 citations in OpenAlex.
- The reduced-charge melittin analogue MelP5 improves the transfection of non-viral DNA nanoparticles.Journal of peptide science : an official publication of the European Peptide Society · 2022Article
- Gene transfection of primary mouse hepatocytes in 384-well plates.Analytical biochemistry · 2022Article
- Functionalized Biodegradable Polymers via Termination of Ring-Opening Polymerization by Acyl Chlorides.Polymers · 2021Article
- Solid-phase supported design of carriers for therapeutic nucleic acid delivery.Bioscience reports · 2017Review
- Efficient expression of stabilized mRNA PEG-peptide polyplexes in liver.Gene therapy · 2015Article
- Miniaturization of gene transfection assays in 384- and 1536-well microplates.Analytical biochemistry · 2015Article
- Dual secured nano-melittin for the safe and effective eradication of cancer cells.Journal of materials chemistry. B · 2015Article
- Engineering biodegradable and multifunctional peptide-based polymers for gene delivery.Journal of biological engineering · 2013Article
- PEG length and chemical linkage controls polyacridine peptide DNA polyplex pharmacokinetics, biodistribution, metabolic stability and in vivo gene expression.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- A Convergent Synthesis of Homogeneous Reducible Polypeptides.Tetrahedron letters · 2013Article
- High-affinity PEGylated polyacridine peptide polyplexes mediate potent in vivo gene expression.Gene therapy · 2013Article
- Exploring the mechanism of plasmid DNA nuclear internalization with polymer-based vehicles.Molecular pharmaceutics · 2012Article
- Metabolically stabilized long-circulating PEGylated polyacridine peptide polyplexes mediate hydrodynamically stimulated gene expression in liver.Gene therapy · 2011Article
- N-glycan targeted gene delivery to the dendritic cell SIGN receptor.Bioconjugate chemistry · 2010Article
- Discovery of metabolically stabilized electronegative polyacridine-PEG peptide DNA open polyplexes.Bioconjugate chemistry · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The combination of a polyacridine peptide modified with a melittin fusogenic peptide results in a potent gene transfer agent. Polyacridine peptides of the general formula (Acr-X)(n)-Cys were prepared by solid-phase peptide synthesis, where Acr is Lys modified on its epsilon-amine with acridine, X is Arg, Leu, or Lys and n is 2, 3, or 4 repeats. The Cys residue was modified by either a maleimide-melittin or a thiolpyridine-Cys-melittin fusogenic peptide resulting in reducible or non-reducible polyacridine-melittin peptides. Hemolysis assays established that polyacridine-melittin peptides retained their membrane lytic potency relative to melittin at pH 7.4 and 5. When combined with plasmid DNA, the membrane lytic potency of polyacridine-melittin peptides was neutralized. Gene transfer experiments in multiple cell lines established that polyacridine-melittin peptides mediate expression as efficiently as PEI. The expression was very dependent upon a disulfide bond linking polyacridine to melittin. The gene transfer was most efficient when X is Arg and n is 3 or 4 repeats. These studies establish polyacridine peptides as a novel DNA binding anchor peptide.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.