ArticleMolecular biology reports2010
Design, expression and characterization of recombinant hybrid peptide Attacin-Thanatin in Escherichia coli.
Article in Molecular biology reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 14 citations in OpenAlex.
- Extracellular production of antifungal peptides from oxidative endotoxin-free E. coli and application.Applied microbiology and biotechnology · 2024Article
- Wnt Signaling Pathway Collapse upon β-Catenin Destruction by a Novel Antimicrobial Peptide SKACP003: Unveiling the Molecular Mechanism and Genetic Activities Using Breast Cancer Cell Lines.Molecules (Basel, Switzerland) · 2023Article
- Cloning and expression analyses of aBioengineered · 2021Article
- Thanatin: An Emerging Host Defense Antimicrobial Peptide with Multiple Modes of Action.International journal of molecular sciences · 2021Review
- Features and applications of Ent35-MccV hybrid bacteriocin: current state and perspectives.Applied microbiology and biotechnology · 2020Review
- Expression of Thanatin in HEK293 Cells and Investigation of its Antibacterial Effects on Some Human Pathogens.Protein and peptide letters · 2020Article
- Identification of immunity-related genes in the larvae of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) by a next-generation sequencing-based transcriptome analysis.Journal of insect science (Online) · 2015Article
- Design and high-level expression of a hybrid antimicrobial peptide LF15-CA8 in Escherichia coli.Journal of industrial microbiology & biotechnology · 2014Article
- Enhanced antibacterial activity of an attacin-coleoptericin hybrid protein fused with a helical linker.Molecular biology reports · 2013Article
- Characterization and expression of attacin, an antibacterial protein-encoding gene, from the beet armyworm, Spodoptera exigua (Hübner) (Insecta: Lepidoptera: Noctuidae).Molecular biology reports · 2012Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antimicrobial peptides will be attractive and potential candidates as peptide drugs because of their efficient action against microbes and low toxicity to mammal cells. To improve their antibacterial activity, some modifications needs to be made. In this research, the hybrid peptide gene Attacin-Thanatin with 642 bp in length with preferred codons of E. coli was generated using the technology of Gene splicing by overlap extension. The gene was inserted in-frame into E. coli expression plasmid pET-32a (+) and induced to express in E. coli Rosetta. The recombinant protein was partial purified and its biological activity was determined. Analysis of the E. coli Rosetta induced with IPTG revealed that the molecular weight of fusion protein was approximately 41.8 kDa, which perfectly matched the mass calculated from the amino acid sequence. Biological activity detection showed that this peptide effectively inhibited the growth of the test bacteria including E. coli DH5α, E. coli BL21 (DE3), Salmonella choleraesuis and Staphylococcus aureus. Among these bacteria, the Gram-negative E. coli was the most sensitive. Furthermore, there was minor hemolysis activity for porcine red blood cells. So, the results indicated that the hybrid peptide Attacin-Thanatin could be served as a promising candidate for the chemical antibiotics.
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